Rabbit anti-Human PEPCK2 Polyclonal Antibody | anti-PCK2 antibody

PEPCK2, NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial), PEPCK-2, PEPCK, PCK2, Mitochondrial Phosphoenolpyruvate Carboxykinase 2, Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial, PEPCK-M, PEP Carboxykinase, Phosphoenolpyruvate Carboxylase)

Cat. Num. AAA623689

• Gene Names: PCK2; PEPCK; PEPCK2; PEPCK-M
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PEPCK2; Polyclonal Antibody; NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial); PEPCK-2; PEPCK; PCK2; Mitochondrial Phosphoenolpyruvate Carboxykinase 2; Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial; PEPCK-M; PEP Carboxykinase; Phosphoenolpyruvate Carboxylase); Anti -PEPCK2; anti-PCK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCK2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PCK2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human PCK2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PCK2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PCK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PCK2 antibody

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-PCK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 66346723
• NCBI GeneID: 5106
• NCBI Accession #: NP_001018083.1
• NCBI GenBank Nucleotide #: NM_001018073.1
• UniProt Accession #: Q16822; O43253; Q86U01; Q9BV62
• Molecular Weight: 70,730 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• NCBI Official Symbol: PCK2
• NCBI Official Synonym Symbols: PEPCK; PEPCK2; PEPCK-M
• NCBI Protein Information: phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEP carboxykinase; phosphopyruvate carboxylase; phosphoenolpyruvate carboxylase
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
• Protein Family: Protein kinase
• UniProt Gene Name: PCK2
• UniProt Synonym Gene Names: PEPCK2; PEPCK-M
• UniProt Entry Name: PCKGM_HUMAN

NCBI Description

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.1.32; Kinase, other; Mitochondrial; Carbohydrate Metabolism - pyruvate; Lyase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; GTP binding; phosphoenolpyruvate carboxykinase (GTP) activity; metal ion binding; phosphoenolpyruvate carboxykinase activity

Biological Process: NADH oxidation; oxaloacetate metabolic process; response to glucocorticoid stimulus; carbohydrate metabolic process; positive regulation of insulin secretion; glucose metabolic process; pathogenesis; pyruvate metabolic process; gluconeogenesis

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial