Goat anti-Human SNF1LK2 Polyclonal Antibody | anti-SIK2 antibody

SNF1LK2 (SNF1-like kinase 2, DKFZp434K1115, KIAA0781, LOH11CR1I, QIK, Qin-induced kinase, Serine/threonine-protein kinase SNF1-like kinase 2, SIK2)

Cat. Num. AAA623060

• Gene Names: SIK2; QIK; SNF1LK2; LOH11CR1I
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SNF1LK2; Polyclonal Antibody; SNF1LK2 (SNF1-like kinase 2; DKFZp434K1115; KIAA0781; LOH11CR1I; QIK; Qin-induced kinase; Serine/threonine-protein kinase SNF1-like kinase 2; SIK2); Anti -SNF1LK2 (SNF1-like kinase 2; anti-SIK2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous human SNF1LK2 at 125kD using Western blots.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute in PBS containing 0.02% sodium azide.

• Applicable Applications for anti-SIK2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Purified, E. coli-derived recombinant human SNF1LK2 (rhSNF1LK2; aa 663-818; Accession# Q9H0K1).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SIK2 antibody

SNF1LK2 (Sucrose non-fermenting 1 like kinase 2) also known as SIK2 (Salt-inducible kinase 2) is a cytoplasmic 125kD member of the CAMK Ser/Thr protein kinase family, AMPK subfamily of enzymes.It is expressed in preadipocytes/adipocytes, with levels strongly induced during early differentiation.SNF1LK2 regulates the early phase of insulin signaling by phosphorylating IRS-1 on Ser794.Human SNF1LK2 is 926 amino acids (aa) in length and contains a protein kinase domain (aa 20-271) with an ATP binding site (Lys49) and a catalytic loop (aa 140-149), followed by a phosphorylation activation site at Thr175, a UBA (ubiquitin-associated) domain (aa 295-335) and another PKA-dependent phosphorylation site at Ser587.

Product Categories/Family for anti-SIK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38569460
• NCBI GeneID: 23235
• NCBI Accession #: NP_056006.1
• NCBI GenBank Nucleotide #: NM_015191.1
• UniProt Accession #: Q9H0K1; O94878; Q17RV0; Q6AZE2; Q76N03; Q8NCV7; Q96CZ8; B0YJ94
• Molecular Weight: 103,915 Da
• NCBI Official Full Name: serine/threonine-protein kinase SIK2
• NCBI Official Synonym Full Names: salt-inducible kinase 2
• NCBI Official Symbol: SIK2
• NCBI Official Synonym Symbols: QIK; SNF1LK2; LOH11CR1I
• NCBI Protein Information: serine/threonine-protein kinase SIK2; SIK-2; SNF1-like kinase 2; qin-induced kinase; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine-protein kinase SNF1-like kinase 2
• UniProt Protein Name: Serine/threonine-protein kinase SIK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SIK2
• UniProt Synonym Gene Names: KIAA0781; QIK; SNF1LK2; SIK-2
• UniProt Entry Name: SIK2_HUMAN

Uniprot Description

QIK: a serine/threonine kinase of the CAMKL family and related to AMPK. Specifically expressed in adipose tissues. Like AMPK, QIK is phosphorylated and activated by LKB1. Part of a molecular complex including TORC2 and calcineurin that regulates the effects of circulating glucose and gut hormones during feeding on TORC2-mediated gene expression. In mice, insulin promotes the phosphorylation and ubiquitin-dependent degradation of TORC2, inhibiting gluconeogenic gene expression during re-feeding. In response to insulin, Akt2 phosphorylates and activates QIK which in turn stimulates the phosphorylation and cytoplasmic translocation of TORC2 where it associates with COP1, which promotes its ubiquitination and proteasomal degradation. Phosphorylates Ser794 of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: regulation of insulin receptor signaling pathway; protein amino acid autophosphorylation; insulin receptor signaling pathway; protein amino acid phosphorylation