Sheep anti-Human Neuroglycan C Polyclonal Antibody
Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5)
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography
Synonyms
Neuroglycan C; Polyclonal Antibody; Neuroglycan C (Chondroitin Sulfate Proteoglycan 5; Acidic Leucine-rich EGF-like Domain-containing Brain Protein; CSPG5); Anti -Neuroglycan C (Chondroitin Sulfate Proteoglycan 5; anti-Neuroglycan C antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Neuroglycan C. Shows approximately 50% cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Form/Format
Supplied as a powder lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 0.5ml sterile PBS.
Applicable Applications for anti-Neuroglycan C antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Immunocytochemistry and Western Blot.
Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Neuroglycan C is approximately 0.3ng/well.
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml was used to detect Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT, After blocking, cells were incubated with diluted C5067-91B for 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Neuroglycan C is approximately 0.3ng/well.
Western Blot: 1ug/ml
Immunocytochemistry: 10ug/ml was used to detect Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT, After blocking, cells were incubated with diluted C5067-91B for 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Immunogen
CHO cell-derived, recombinant human Neuroglycan C extracellular domain (aa 31 - 420; Accession # AAQ04776)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Neuroglycan C antibody
Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120-160kD member of the neuregulin family of proteins. Depending upon its expression profile, it may be a glycoprotein of 120kD, or a chondroitin sulfate (CS) proteoglycan of 140-160kD. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons. Mature human NGC is a 536 amino acid (aa) type I transmembrane glycoprotein. It possesses a 393 aa extracellular region (aa31-423) that contains a Ser117 CS attachment site and an EGF-like domain (aa 371-413). There are at least two isoform variants. One shows a deletion of aa 487-513, while a second shows an alternaive start site at Met139 with the same deletion. Phosphorylation likely occurs on Ser249, and proteolysis generates a >75kD soluble fragment. Over aa 31-420, human NGC shares 84% aa identity with mouse NGC.
Product Categories/Family for anti-Neuroglycan C antibody
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a proteoglycan that may function as a neural growth and differentiation factor. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Similar Products
Product Notes
The Neuroglycan C (Catalog #AAA622669) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Neuroglycan C (Chondroitin Sulfate Proteoglycan 5, Acidic Leucine-rich EGF-like Domain-containing Brain Protein, CSPG5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neuroglycan C can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in ELISA, Immunocytochemistry and Western Blot. Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Neuroglycan C is approximately 0.3ng/well. Western Blot: 1ug/ml Immunocytochemistry: 10ug/ml was used to detect Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT, After blocking, cells were incubated with diluted C5067-91B for 3 hours at RT followed by NorthernLights 557 donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Researchers should empirically determine the suitability of the Neuroglycan C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuroglycan C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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