Rabbit AKT1 Polyclonal Antibody | anti-AKT1 antibody

AKT1 (v-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) (MaxLight 550)

Cat. Num. AAA6461991

• Gene Names: Akt1; Akt
• Reactivity: Human, Mouse, Rat
• Applications: Immunoblot, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: AKT1; Polyclonal Antibody; AKT1 (v-akt Murine Thymoma Viral Oncogene Homolog 1; C-AKT; MGC99656; PKB; PRKBA; Protein kinase B; RAC; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha) (MaxLight 550); anti-AKT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human, rat and mouse Akt1/PKBa, Mr 60kD. May crossreact with human Akt2 but not with Akt3.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-AKT1 antibody: Immunoblot (IB)
• Application Notes: Immunoblot: 0.5-2ug/ml detects Akt in RIPA lysates from human A431 carcinoma; Applications are based on unconjugated antibody.
• Immunogen: 15 residue synthetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1.
• Conjugate: MaxLight550
• Included Positive Antigen Control: Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: A431 cell lysate was probed with Anti-Akt1/PKBalpha)

Related Product Information for anti-AKT1 antibody

A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Akt1/PKBa (2ug/ml). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Product Categories/Family for anti-AKT1 antibody

Antibodies; Protein Kinases

References

1. Cross, D.A., et al., Nature 378: 785-789, 1995. 2. James, S.R., et al., Biochem. J. 315: 709-713, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 15100164
• NCBI GeneID: 24185
• NCBI Accession #: NP_150233.1
• NCBI GenBank Nucleotide #: NM_033230.2
• UniProt Accession #: P47196
• Molecular Weight: 55,735 Da
• NCBI Official Full Name: RAC-alpha serine/threonine-protein kinase
• NCBI Official Synonym Full Names: AKT serine/threonine kinase 1
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: Akt
• NCBI Protein Information: RAC-alpha serine/threonine-protein kinase
• UniProt Protein Name: RAC-alpha serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: PKB; PKB alpha

NCBI Description

inhibits JUN kinase activation and mediates inhibition of apoptosis [RGD, Feb 2006]

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: AGC group; AKT family; EC 2.7.11.1; Kinase, protein; Oncoprotein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 6q32

Cellular Component: cytoplasm; cytosol; intercellular junction; microtubule cytoskeleton; mitochondrion; nucleoplasm; nucleus; plasma membrane; protein complex; spindle; vesicle

Molecular Function: 14-3-3 protein binding; ATP binding; enzyme binding; GTPase activating protein binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein kinase C binding; protein phosphatase 2A binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: activated T cell apoptosis; aging; apoptotic mitochondrial changes; cell projection organization; cellular response to cadmium ion; cellular response to DNA damage stimulus; cellular response to epidermal growth factor stimulus; cellular response to growth factor stimulus; cellular response to hypoxia; cellular response to insulin stimulus; cellular response to organic cyclic compound; cellular response to peptide; cellular response to prostaglandin E stimulus; cellular response to reactive oxygen species; cellular response to tumor necrosis factor; epidermal growth factor receptor signaling pathway; establishment of protein localization to mitochondrion; germ cell development; glucose homeostasis; glucose metabolic process; glucose transport; glycogen biosynthetic process; glycogen metabolic process; hyaluronan metabolic process; I-kappaB kinase/NF-kappaB signaling; inflammatory response; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; interleukin-7-mediated signaling pathway; lipopolysaccharide-mediated signaling pathway; maintenance of protein location in mitochondrion; maternal placenta development; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of cell size; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of endopeptidase activity; negative regulation of fatty acid beta-oxidation; negative regulation of gene expression; negative regulation of JNK cascade; negative regulation of plasma membrane long-chain fatty acid transport; negative regulation of protein kinase activity; negative regulation of proteolysis; negative regulation of release of cytochrome c from mitochondria; NIK/NF-kappaB signaling; osteoblast differentiation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; peripheral nervous system myelin maintenance; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; phosphorylation; platelet activation; positive regulation of apoptosis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle; positive regulation of endodeoxyribonuclease activity; positive regulation of endothelial cell proliferation; positive regulation of fat cell differentiation; positive regulation of fibroblast migration; positive regulation of glucose import; positive regulation of glucose metabolic process; positive regulation of glycogen biosynthetic process; positive regulation of lipid biosynthetic process; positive regulation of mitochondrial membrane potential; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein localization to nucleus; positive regulation of protein phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of sodium ion transport; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of vasoconstriction; protein amino acid phosphorylation; protein catabolic process; protein import into nucleus, translocation; protein kinase B signaling; protein ubiquitination; regulation of apoptosis; regulation of cell migration; regulation of glycogen biosynthetic process; regulation of myelination; regulation of neuron projection development; regulation of protein localization; regulation of translation; response to food; response to hormone; response to insulin-like growth factor stimulus; response to organic substance; response to oxidative stress; signal transduction; spinal cord development; striated muscle cell differentiation; translation