Sheep anti-Human Methionine Aminopeptidase 2 Polyclonal Antibody | anti-METAP2 antibody

Methionine Aminopeptidase 2 (MetAP-2)

Cat. Num. AAA621880

• Gene Names: METAP2; p67; MAP2; MNPEP; p67eIF2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Methionine Aminopeptidase 2; Polyclonal Antibody; Methionine Aminopeptidase 2 (MetAP-2); Anti -Methionine Aminopeptidase 2 (MetAP-2); anti-METAP2 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human METAP2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.

• Applicable Applications for anti-METAP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunoprecipitation.; Dilution: ELISA: 0.5-1ug/ml; Western Blot: 1ug/ml
• Immunogen: Sf 21-derived, recombinant human Methionine Aminopeptidase 2 (aa2-478).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of METAP2 with MBS621880. Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0ug/mL sheep anti-hMETAP2.)

Related Product Information for anti-METAP2 antibody

In vitro, the metalloprotease MetAP-2 catalyzes the hydrolysis of N-terminal methionine residues. MetAP-2 is highly conserved across species. The catalytic domains of S. cerevisiae and human MetAP-2 proteins are ~80% homologous. The function of MetAP-2 in vivo remains to be determined. MetAP-2 has been identified, purified and cloned from rat cells as a 67kD eIF-2-associated (elongation inhibitory factor-2) protein, suggesting a possible role in protein translation. Provocative experiments suggest MetAP-2 may also be involved in angiogenesis, the process of new blood vessel formation. In these studies, MetAP-2 was determined to be the major cellular protein covalently bound to the potent anti-angiogenic agent fumagillin, following fumagillin treatment of HUVEC (human umbilical endothelial cells) cells or calf brain homogenates. Fumagillin was able to selectively inhibit the growth of a MetAP-2-dependent yeast strain. The fungal derived fumagillin, and its more potent metabolite TNP-470 (now in clinical trials), are effective at limiting both the size and metastasis of solid tumors. TNP-470 inhibits neovascularization via endothelial cell cycle arrest in the late G1 phase. The details of this process are not well understood. In view of the identification of MetAP-2 as a fumagillin binding protein, future studies with MetAP-2 should provide important new insights into the mechanisms of fumagillin/TNP- 470 induced cell-cycle arrest and the role of MetAP-2 in angiogenesis.

Product Categories/Family for anti-METAP2 antibody

Antibodies; Abs to Amino Acids

NCBI and Uniprot Product Information

• NCBI GI #: 5803092
• NCBI GeneID: 10988
• NCBI Accession #: NP_006829.1
• NCBI GenBank Nucleotide #: NM_006838.3
• UniProt Accession #: P50579; B2RDI8; Q8NB11; Q96B43
• Molecular Weight: 52,892 Da
• NCBI Official Full Name: methionine aminopeptidase 2
• NCBI Official Synonym Full Names: methionyl aminopeptidase 2
• NCBI Official Symbol: METAP2
• NCBI Official Synonym Symbols: p67; MAP2; MNPEP; p67eIF2
• NCBI Protein Information: methionine aminopeptidase 2; MAP 2; metAP 2; peptidase M 2; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein
• UniProt Protein Name: Methionine aminopeptidase 2
• Protein Family: Methionine aminopeptidase
• UniProt Gene Name: METAP2
• UniProt Synonym Gene Names: MNPEP; P67EIF2
• UniProt Entry Name: AMPM2_HUMAN

NCBI Description

This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Uniprot Description

METAP2: Removes the N-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Interacts strongly with the eIF-2 gamma-subunit EIF2S3. Binds EIF2S1 at low magnesium concentrations. Belongs to the peptidase M24A family.

Protein type: Protease; EC 3.4.11.18

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; peptidyl-methionine modification; protein processing; proteolysis; N-terminal protein amino acid modification