Goat anti-Human Urokinase Plasminogen Activator Receptor Polyclonal Antibody | anti-PLAUR antibody

Urokinase Plasminogen Activator Receptor (uPA-R)

Cat. Num. AAA621849

• Gene Names: PLAUR; CD87; UPAR; URKR; U-PAR
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: Urokinase Plasminogen Activator Receptor; Polyclonal Antibody; Urokinase Plasminogen Activator Receptor (uPA-R); Anti -Urokinase Plasminogen Activator Receptor (uPA-R); anti-PLAUR antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Urokinase Plasminogen Activator-Receptor. There were no cross reactivities obtained with human tPA and human PAI-2.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.

• Applicable Applications for anti-PLAUR antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry.; Dilution: Immunohistochemistry: 1:50-1:100. Frozen sections & paraffin sections. Pretreatment with; 0.05% Pronase E for 20 minutes.
• Immunogen: Purified human Urokinase Plasminogen Activator-Receptor
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PLAUR antibody

The urokinase-type plasminogen activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity.

The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. Mouse uPAR-1/Fc cDNA encodes a 327 amino acid residue precursor protein with a 23 aa residue signal peptide, seven potential N-linked glycosylation sites and a C-terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% amino acid sequence identity and the receptor-ligand interaction is highly species-specific.

Product Categories/Family for anti-PLAUR antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 433901
• NCBI GeneID: 5329
• UniProt Accession #: Q03405; Q12876; Q15845; Q16887; Q6IB52; Q9BWT0; Q9NYC8; Q9UD69; Q9UEA6; Q9UM92; Q9UMV0; A8K409; Q6GY32; Q9UPI5
• Molecular Weight: 36,978 Da
• NCBI Official Full Name: urokinase plasminogen activator receptor
• NCBI Official Synonym Full Names: plasminogen activator, urokinase receptor
• NCBI Official Symbol: PLAUR
• NCBI Official Synonym Symbols: CD87; UPAR; URKR; U-PAR
• NCBI Protein Information: urokinase plasminogen activator surface receptor; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2
• UniProt Protein Name: Urokinase plasminogen activator surface receptor
• Protein Family: Urokinase plasminogen activator surface receptor
• UniProt Gene Name: PLAUR
• UniProt Synonym Gene Names: MO3; UPAR
• UniProt Entry Name: UPAR_HUMAN

NCBI Description

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq]

Uniprot Description

uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: endoplasmic reticulum membrane; focal adhesion; extrinsic to membrane; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane

Molecular Function: protein domain specific binding; protein binding; enzyme binding; U-plasminogen activator receptor activity; receptor activity; receptor binding

Biological Process: fibrinolysis; cellular protein metabolic process; attachment of GPI anchor to protein; regulation of proteolysis; C-terminal protein lipidation; blood coagulation; signal transduction; post-translational protein modification; chemotaxis; cell motility