Goat anti-Human Indoleamine 2,3-Dioxygenase-like 1 protein Polyclonal Antibody | anti-IDO2 antibody

Indoleamine 2,3-Dioxygenase-like 1 protein (INDOL1, IDO2, indoleamine 2,3-dioxygenase 2, indoleamine-pyrrole 2,3 dioxygenase-like 1)

Cat. Num. AAA621840

• Gene Names: IDO2; INDOL1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Indoleamine 2; 3-Dioxygenase-like 1 protein; Polyclonal Antibody; 3-Dioxygenase-like 1 protein (INDOL1; IDO2; indoleamine 2; 3-dioxygenase 2; indoleamine-pyrrole 2; 3 dioxygenase-like 1); Anti -Indoleamine 2; anti-IDO2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human INDOL1. Species Sequence Homology: canine, bovine
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-IDO2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA: 1:64,000; Western Blot: 1-3ug/ml detects ~50kD band in human kidney lysates.
• Immunogen: Synthetic peptide corresponding to C-RDKTLESILHPR from the C-Terminus of human Indoleamine 2,3-Dioxygenase-like 1 protein (INDOL1) (Accession #NP_919270.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS621840 (0.5ug/ml) staining of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-IDO2 antibody

Indoleamine 2,3-dioxygenase (IDO), a monomeric hemoprotein, is the initial and rate-limiting enzyme of the kynurenine pathway of degradation of L-tryptophan. IDO oxidizes the pyrrole moiety of tryptophan, causing cell death due to tryptophan starvation. It is induced in various types of human cell lines and cell types such as fibroblasts, epithelial cells and macrophages in the presence of IFN-gamma. Placenta, lung and small intestine show high levels of IDO enzyme activity. IDO has been implicated in regulation of feto-maternal tolerance and protection against intracellular and extracellular pathogens. In the human female reproductive tract, endometrial glandular and surface epithelial cells show increasing IDO expression during the course of the menstrual cycle. IDO is also implicated in playing immuno-regulation. In vivo, IDO has been implicated in tolerance to self antigens in non-obese diabetic mice, and as a downstream effector mechanism for the toleance-inducing agent CTLA4-Ig. Expression of IDO by tumor cells has also been proposed as a mechanism by which tumors evade the immune system. Consistent with this hypothesis, a recent study in mice found a subset of plasmacytoid deudritic cells (pDCs) in tumor-draining lymph nodes (TDLNs) that expressed immunosuppressive levels of IDO.

Product Categories/Family for anti-IDO2 antibody

Antibodies; Abs to Enzymes; Oxidase

NCBI and Uniprot Product Information

• NCBI GI #: 148539554
• NCBI GeneID: 169355
• NCBI Accession #: NP_919270.2
• NCBI GenBank Nucleotide #: NM_194294.2
• UniProt Accession #: Q6ZQW0; A4UD41
• Molecular Weight: 45,424 Da
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 2
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 2
• NCBI Official Symbol: IDO2
• NCBI Official Synonym Symbols: INDOL1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 2; IDO-2; indoleamine 2,3-dioxygenase-like 1 protein; indoleamine 2,3-dioxygenase-like protein 1; indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine-pyrrole 2,3-dioxygenase-like protein 1
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 2
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: IDO2
• UniProt Synonym Gene Names: INDOL1; IDO-2
• UniProt Entry Name: I23O2_HUMAN

NCBI Description

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011]

Uniprot Description

INDOL1: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Belongs to the indoleamine 2,3-dioxygenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Amino Acid Metabolism - tryptophan; EC 1.13.11.-

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: cytoplasm; cytosol

Molecular Function: metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; tryptophan 2,3-dioxygenase activity

Biological Process: tryptophan catabolic process to kynurenine; tryptophan catabolic process