Goat MRP4 Polyclonal Antibody | anti-ABCC4 antibody

MRP4 (Multidrug Resistance-associated Protein 4, ATP-binding Cassette Sub-family C (CFTR/MRP) Member 4, ATP Binding Cassette Sub-family C Member 4, ABCC4, bA464I2.1, Canalicular Multispecific Organic Anion Transporter ABC Superfamily, Canalicular Multispe

Cat. Num. AAA621820

• Gene Names: ABCC4; MRP4; MOATB; MOAT-B; EST170205
• Reactivity: Human, Monkey, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MRP4; Polyclonal Antibody; MRP4 (Multidrug Resistance-associated Protein 4; ATP-binding Cassette Sub-family C (CFTR/MRP) Member 4; ATP Binding Cassette Sub-family C Member 4; ABCC4; bA464I2.1; Canalicular Multispecific Organic Anion Transporter ABC Superfamily; Canalicular Multispe; Anti -MRP4 (Multidrug Resistance-associated Protein 4; anti-ABCC4 antibody

• Host: Goat
• Reactivity: Human, Monkey, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ABCC4 at ~170kD in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kD according to Human NP_005836.2). This antibody is expected to recognize isoform 1 (NP_005836.2) only. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline 0.5mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ABCC4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry.; Dilution: ELISA: 1:64000; Western Blot:1-3ug/ml
• Immunogen: Synthetic peptide of ABCC4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ABCC4 in rat renal cortex membranes (20μg total protein per lane) using ABCC4 antibody (0.5μg/ml).)

Immunohistochemistry (IHC)

(ABCC4 staining of paraffin embedded Human Prostate using ABCC4 antibody at 2.5μg/ml.)

Related Product Information for anti-ABCC4 antibody

Proteins involved in MDR mechanisms are P-glycoprotein (Pgp), Multidrug Resistance-associated Protein (MRP) and Lung Resistance-related Protein (LRP). Pgp and MRP belong to the ATP binding cassette (ABC) superfamily of membrane transporter proteins. This superfamily comprises a broad range of proteins found in organisms from bacteria to humans, which transport a wide variety of substances such as ions, amino acids, sugars, peptide and proteins.

Product Categories/Family for anti-ABCC4 antibody

Antibodies; Abs to Multi Drug Resistance (MDR)

NCBI and Uniprot Product Information

• NCBI GI #: 157502203
• NCBI GeneID: 10257
• NCBI Accession #: NP_001098985.1
• NCBI GenBank Nucleotide #: NM_001105515.1
• UniProt Accession #: Q8IVZ4; O15439; O75555; Q59GY6; Q8IUA3; Q8IWU1
• Molecular Weight: 96,841 Da
• NCBI Official Full Name: multidrug resistance-associated protein 4 isoform 2
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 4
• NCBI Official Symbol: ABCC4
• NCBI Official Synonym Symbols: MRP4; MOATB; MOAT-B; EST170205
• NCBI Protein Information: multidrug resistance-associated protein 4; OTTHUMP00000018560; MRP/cMOAT-related ABC transporter; multispecific organic anion transporter B; ATP-binding cassette sub-family C member 4; multi-specific organic anion transporter B; bA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4); canalicular multispecific organic anion transporter (ABC superfamily)
• UniProt Protein Name: ABCC4 protein
• Protein Family: Multidrug resistance-associated protein
• UniProt Gene Name: ABCC4
• UniProt Entry Name: Q8IVZ4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

ABCC4: a widely-expressed multi-pass membrane protein. An organic anion pump involved in cellular detoxification. Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: membrane; basolateral plasma membrane; integral to membrane; plasma membrane; platelet dense granule membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; ATP binding

Biological Process: response to drug; platelet activation; platelet degranulation; positive regulation of smooth muscle cell proliferation; blood coagulation; response to organic nitrogen; transmembrane transport; response to organic cyclic substance