SDS-Page
(Detection of SPRY3. Lysates from human HepG2, MCF-7 and CaCo-2 cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1.0ug/ml anti-SPRY3, as described in Protocols for Immunoblotting.)
Sheep anti-Human Sprouty 3 Polyclonal Antibody | anti-SPRY3 antibody
Sprouty 3 (SPRY3, Sprouty homolog 3 (Drosophila) HSPRY3, Sprouty homolog 3, Spry-3)
Gene Names
SPRY3; HSPRY3
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Sprouty 3; Polyclonal Antibody; Sprouty 3 (SPRY3; Sprouty homolog 3 (Drosophila) HSPRY3; Sprouty homolog 3; Spry-3); Anti -Sprouty 3 (SPRY3; anti-SPRY3 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SPRY3 at ~42kD.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS,5% trehalose. Reconstitute with 40-50% glycerol, PBS.
Applicable Applications for anti-SPRY3 antibody
Western Blot (WB)
Application Notes
Dilution: Western Blot: 1.0ug/ml
Immunogen
Recombinant human SPRY3 aa1-142; O43610 (E. coli).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of SPRY3. Lysates from human HepG2, MCF-7 and CaCo-2 cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1.0ug/ml anti-SPRY3, as described in Protocols for Immunoblotting.)
SDS-Page
(Detection of SPRY3. Lysates from human HepG2, MCF-7 and CaCo-2 cells were resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1.0ug/ml anti-SPRY3, as described in Protocols for Immunoblotting.)
Related Product Information for anti-SPRY3 antibody
SPRY3 (sprouty homolog 3) is a member of the sprouty family of proteins. SPRY3 appears to modulate Ras/MAPK pathway signaling following RTK activation. Human SPRY3 is 288aa in length and contains one SH2-binding domain (aa 25-30), a PEST sequence (aa82-93), and a Cys-rich domain (aa147-270). SPRY3 forms homo- and hetero-oligomers with other SPRY family molecules. It also undergoes phosphorylation, ubiquitination and palmitoylation, the latter of which induces SPRY3 to associate with cell membranes.
Product Categories/Family for anti-SPRY3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,222 Da
NCBI Official Full Name
sprouty (Drosophila) homolog 3
NCBI Official Synonym Full Names
sprouty homolog 3 (Drosophila)
NCBI Official Symbol
SPRY3
NCBI Official Synonym Symbols
HSPRY3
NCBI Protein Information
protein sprouty homolog 3; spry-3; OTTHUMP00000024256; antagonist of FGF signaling
UniProt Protein Name
Protein sprouty homolog 3
Protein Family
UniProt Gene Name
SPRY3
UniProt Entry Name
SPY3_HUMAN
Uniprot Description
SPRY3: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Belongs to the sprouty family.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: Xq28 and Yq12
Cellular Component: membrane; cytoplasm
Biological Process: multicellular organismal development; regulation of signal transduction
Research Articles on SPRY3
Similar Products
Product Notes
The SPRY3 spry3 (Catalog #AAA621283) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Sprouty 3 (SPRY3, Sprouty homolog 3 (Drosophila) HSPRY3, Sprouty homolog 3, Spry-3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sprouty 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Dilution: Western Blot: 1.0ug/ml. Researchers should empirically determine the suitability of the SPRY3 spry3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sprouty 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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