Rabbit anti-Mouse Adrenergic Receptor, beta 3 Polyclonal Antibody | anti-ADRB3 antibody

Adrenergic Receptor, beta 3 (B3AR, BAR3, ADRB3R, Beta-3 adrenoreceptor)

Cat. Num. AAA620379

• Gene Names: ADRB3; BETA3AR; FLJ99960
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Adrenergic Receptor; beta 3; Polyclonal Antibody; beta 3 (B3AR; BAR3; ADRB3R; Beta-3 adrenoreceptor); Anti -Adrenergic Receptor; anti-ADRB3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Adrenergic Receptor, beta 3 (B3AR).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

• Applicable Applications for anti-ADRB3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:50,000. Using 50-100ng control peptide/well.
• Immunogen: Synthetic peptide corresponding to 20aa at the C-terminus of mouse B3AR (KLH). Species Sequence Homology: rat, 85%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ADRB3 antibody

The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptor superfamily. They are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs. This stimulation in turn activates adenyl cyclase. Activation of adenyl cyclase yields the second messenger cAMP. B3AR is primarily present in adipocytes The B3AR plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In humans, B3AR, a Trp to Arg replacement has recently been discovered at position 64. This change in the first coding exon has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). Chronic stimulation of the B3AR by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle. This expression may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408aa membrane protein (rat/mouse, 400aa). The N-terminus is predicted to be extracellular; the C-terminus intracellular.

Product Categories/Family for anti-ADRB3 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 40037473
• NCBI GeneID: 155
• UniProt Accession #: P13945; Q4JFT4
• Molecular Weight: 43,519 Da
• NCBI Official Full Name: adrenergic receptor beta 3
• NCBI Official Synonym Full Names: adrenergic, beta-3-, receptor
• NCBI Official Symbol: ADRB3
• NCBI Official Synonym Symbols: BETA3AR; FLJ99960
• NCBI Protein Information: beta-3 adrenergic receptor; OTTHUMP00000225623; beta-3 adrenoceptor; beta-3 adrenoreceptor
• UniProt Protein Name: Beta-3 adrenergic receptor
• Protein Family: Beta-3 adrenergic receptor
• UniProt Gene Name: ADRB3
• UniProt Synonym Gene Names: ADRB3R; B3AR
• UniProt Entry Name: ADRB3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq]

Uniprot Description

ADRB3: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: integral to plasma membrane; plasma membrane; nucleus; receptor complex

Molecular Function: beta3-adrenergic receptor activity; protein binding; protein homodimerization activity; beta-3 adrenergic receptor binding; norepinephrine binding; epinephrine binding; beta-adrenergic receptor activity

Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; generation of precursor metabolites and energy; diet induced thermogenesis; negative regulation of multicellular organism growth; eating behavior; adenylate cyclase activation; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; response to antibiotic; G-protein signaling, coupled to cyclic nucleotide second messenger; positive regulation of MAPKKK cascade; heat generation; carbohydrate metabolic process; brown fat cell differentiation; energy reserve metabolic process; response to cold; aging; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure

Disease: Obesity