Rabbit anti-Human CDC25C Polyclonal Antibody

CDC25C, phosphorylated (Ser216) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)

Cat. Num. AAA619574

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: CDC25C; Polyclonal Antibody; phosphorylated (Ser216) (Cell Division Cycle 25 Homolog C; Cdc 25C; CDC 25; Dual Specificity Phosphatase Cdc25C; M-phase Inducer Phosphatase 3; MPIP3; PPP1R60); Anti -CDC25C; anti-CDC25C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human cdc25C at ~60kD only when phosphorylated at Ser216.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-CDC25C antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of lysates from control (Cont.), nocodazole (Ncz.) and UV (UV) treated HT29 cell lysates, using MBS619574 (upper) and cdc25C Antibody (lower).)

Western Blot (WB)

(Western Blot analysis of control and Chk2 phosphorylated GST, GST-cdc25C and mutant GST-cdc25C Ser216Ala, using MBS619574.)

Western Blot (WB)

(Western Blot analysis of GST-cdc25C phosphorylated by cdc2/cyclin B, Chk2 or both kinases, using MBS619574 (upper) and Phospho-cdc25C (Ser214) Antibody (lower).)

Related Product Information for anti-CDC25C antibody

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).

Product Categories/Family for anti-CDC25C antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 15723723
• NCBI Official Full Name: CDC25C

NCBI Description

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: protein binding; WW domain binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; viral reproduction; cell division; regulation of cell cycle; regulation of mitosis; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; DNA replication; G2/M transition of mitotic cell cycle