Rabbit anti-Human CDC25C Polyclonal Antibody

CDC25C, phosphorylated (Thr48) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)

Cat. Num. AAA614116

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CDC25C; Polyclonal Antibody; phosphorylated (Thr48) (Cell Division Cycle 25 Homolog C; Cdc 25C; CDC 25; Dual Specificity Phosphatase Cdc25C; M-phase Inducer Phosphatase 3; MPIP3; PPP1R60); Anti -CDC25C; anti-CDC25C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-cdc25C (Thr48) Antibody detects endogenous levels of cdc25C phosphorylated at threonine 48.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-CDC25C antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Dilution: Western Blotting: 1:1000; Immunohistochemistry (Paraffin): 1:50; Immunocytochemistry (for IF): 1:100
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr48 of human cdc25C.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from control (cont.) or 50 ng/ml nocodazole treated (M) HT29 cells and of GST-cdc25C nonphosphorylated (-P) or phosphorylated (+P) with cdc2/cyclin B, using MBS614116.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of phosphorylated cdc25C in paraffin-embedded human breast carcinoma showing nuclear localization, using MBS614116.)

Immunohistochemistry (IHC)

(Immunofluorescent detection of control (left) and nocodazole treated (right) HeLa cells, using Immunohistochemical staining of phosphorylated cdc25C in paraffin-embedded human breast carcinoma showing nuclear localization, using MBS614116.(red) and Phospho-Histone H3 (Ser10) (6G3) Monoclonal Antibody (green).)

Related Product Information for anti-CDC25C antibody

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into the M-phase of the cell cycle (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). Ser216- phosphorylated cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm to prevent premature mitosis (3). The checkpoint kinases Chk1 and Chk2 have been shown to phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). Full activation of cdc25C involves phosphorylation at more than 12 different phosphorylation sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C have been shown to interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10).

Product Categories/Family for anti-CDC25C antibody

Antibodies; Abs to Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 15723723
• NCBI Official Full Name: CDC25C

NCBI Description

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytosol; nucleus

Molecular Function: protein binding; WW domain binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: mitosis; cell proliferation; viral reproduction; cell division; regulation of cell cycle; regulation of mitosis; spermatogenesis; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; DNA replication