Mouse ATP6V1B2 Monoclonal Antibody | anti-ATP6V1B2 antibody

ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kD, V1 Subunit B2, ATP6B1B2, ATP6B2, HO57, VATB, VPP3, Vma2) (HRP)

Cat. Num. AAA6182468

• Gene Names: ATP6V1B2; DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2
• Applications: ELISA, Western Blot
• Purity: Purified
• Synonyms: ATP6V1B2; Monoclonal Antibody; ATP6V1B2 (ATPase; H+ Transporting; Lysosomal 56/58kD; V1 Subunit B2; ATP6B1B2; ATP6B2; HO57; VATB; VPP3; Vma2) (HRP); ATPase; Vma2; anti-ATP6V1B2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A5
• Specificity: Recognizes ATP6V1B2.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 511

• Applicable Applications for anti-ATP6V1B2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: ATP6V1B2 (AAH03100, 1aa-511aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MALRAMRGIVNGAAPELPVPTGGPAVGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ATP6V1B2 expression in transfected 293T cell line by ATP6V1B2 monoclonal antibody (M05), clone 2A5.Lane 1: ATP6V1B2 transfected lysate (Predicted MW: 56.4 KDa).Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-ATP6V1B2 antibody

Antibodies; Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 13111865
• NCBI GeneID: 526
• NCBI Official Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit B2
• NCBI Official Symbol: ATP6V1B2
• NCBI Official Synonym Symbols: DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2
• NCBI Protein Information: V-type proton ATPase subunit B, brain isoform
• Protein Family: V-type proton ATPase

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]