Goat Aldehyde Dehydrogenase 1A1 Polyclonal Antibody | anti-ALDH1A1 antibody

Aldehyde Dehydrogenase 1A1 (Aldh1a1, Acetaldehyde Dehydrogenase 1, AHD2, ALDC, Aldehyde Dehydrogenase 1 Soluble, Aldehyde Dehydrogenase 1A1, Aldehyde Dehydrogenase Cytosolic, Aldehyde Dehydrogenase Liver (cytosolic), MGC2318, PUMB1, RALDH 1, RalDH1, Retin

Cat. Num. AAA619382

• Gene Names: ALDH1A1; ALDC; ALDH1; PUMB1; ALDH11; RALDH1; ALDH-E1; MGC2318
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Aldehyde Dehydrogenase 1A1; Polyclonal Antibody; Aldehyde Dehydrogenase 1A1 (Aldh1a1; Acetaldehyde Dehydrogenase 1; AHD2; ALDC; Aldehyde Dehydrogenase 1 Soluble; Aldehyde Dehydrogenase Cytosolic; Aldehyde Dehydrogenase Liver (cytosolic); MGC2318; PUMB1; RALDH 1; RalDH1; Retin; Anti -Aldehyde Dehydrogenase 1A1 (Aldh1a1; anti-ALDH1A1 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ALDH1A1 at ~55kD in human liver lysates (calculated MW of 54.9kD according to NP_000680.2). This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Species Crossreactivity: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ALDH1A1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 0.03-0.1ug/ml; Titer: 1:16,000 (by peptide ELISA)
• Immunogen: Synthetic peptide corresponding to amino acids near the C-terminus of human ALDH1A1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ALDH1A1 in human liver lysate (RIPA buffer, 35μg total protein per lane) using ALDH1A1 antibody (0.03μg/ml).)

Related Product Information for anti-ALDH1A1 antibody

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes.

Product Categories/Family for anti-ALDH1A1 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 62089228
• NCBI GeneID: 216
• UniProt Accession #: Q59EJ0; P00352; Q5SYQ7; Q5SYQ8; Q5SYQ9
• Molecular Weight: 35,780 Da
• NCBI Official Full Name: aldehyde dehydrogenase 1A1 variant
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member A1
• NCBI Official Symbol: ALDH1A1
• NCBI Official Synonym Symbols: ALDC; ALDH1; PUMB1; ALDH11; RALDH1; ALDH-E1; MGC2318
• NCBI Protein Information: retinal dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; OTTHUMP00000021482; acetaldehyde dehydrogenase 1; retinaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic
• UniProt Protein Name: Aldehyde dehydrogenase 1A1 variant
• Protein Family: Retinal dehydrogenase
• UniProt Entry Name: Q59EJ0_HUMAN

NCBI Description

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq]

Uniprot Description

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: Oxidoreductase; Apoptosis; EC 1.2.1.36; Cofactor and Vitamin Metabolism - retinol; GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation