Sheep anti-Human, Mouse Ryk Polyclonal Antibody
Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Ryk; Polyclonal Antibody; Ryk (Related to Tyrosine (Y) Kinase; VIK; MRK); Anti -Ryk (Related to Tyrosine (Y) Kinase; anti-Ryk antibody
Host
Sheep
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Ryk. Species Crossreactivity: human
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-Ryk antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot.
Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.
Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA.
Western Blot: 1ug/ml
Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well.
Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA.
Western Blot: 1ug/ml
Immunogen
NS0-derived, recombinant mouse Ryk, extracellular domain (aa35-211, Accession #Q01887).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Ryk antibody
Ryk (related to tyrosine (Y) kinase; also VIK and MRK) is a member of the tyrosine protein kinase family. It is a type I transmembrane glycoprotein that binds Wnt and forms a Wnt receptor complex with frizzled. In this capacity, it serves as a link between Wnt and Dishevelled. Mouse Ryk is 594 amino acids (aa) in length. It contains a 177 aa extracellular region (aa 35-211) that shows one WIF-1-like domain (aa 51-180), and a cytoplasmic region that possesses a nonfunctional Ser/Thr protein kinase domain (aa 318-581). There is one alternate start site at Met117. Over aa 35-211, mouse Ryk is 94% aa identical to human Ryk, and shows absolute (100%) aa identity to rat Ryk.
Product Categories/Family for anti-Ryk antibody
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Product Notes
The Ryk (Catalog #AAA618858) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Ryk (Related to Tyrosine (Y) Kinase, VIK, MRK) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ryk can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit is ~1ng/well. Immunocytochemistry: 10ug/ml. NIH3T3 cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted R9951-50 followed by NL557-coupled anti-sheep IgG in the dark. Between each step, cells were washed with PBS containing BSA. Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the Ryk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ryk, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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