Western Blot (WB)
(Western Blot using MBS618482 human cells on left, mouse cells on right.)
Rabbit c-erbB2, p185 Polyclonal Antibody
c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free)
Reactivity
Human, Monkey, Mouse, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
c-erbB2; p185; Polyclonal Antibody; p185 (HER2; neu Receptor) (BSA & Azide Free); Anti -c-erbB2; anti-c-erbB2; p185 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Species Crossreactivity: monkey, rat and mouse.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015.
Applicable Applications for anti-c-erbB2, p185 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry.
Dilution: Immunoprecipitation: 10ug/mg protein lysate. Use Protein A.
Western Blot: 5-10ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Dilution: Immunoprecipitation: 10ug/mg protein lysate. Use Protein A.
Western Blot: 5-10ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunogen
Synthetic peptide derived from the C-terminus of human c-erbB-2/HER-2 protein. Sequence is identical in rat neu protein. Cellular Localization: Cell membrane. Epitope: C-terminal.
Positive Control
SKBR-3 cells, T47D cells or breast carcinomas.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot using MBS618482 human cells on left, mouse cells on right.)
Western Blot (WB)
(Western Blot using MBS618482 human cells on left, mouse cells on right.)
Western Blot (WB)
(IP / Westem Blot of human cells using MBS618482)
Western Blot (WB)
(IP / Westem Blot of human cells using MBS618482)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with MBS618482 using peroxidase labeled and AEC chromogen. Note membrane staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with MBS618482 using peroxidase labeled and AEC chromogen. Note membrane staining of tumor cells.)
Related Product Information for anti-c-erbB2, p185 antibody
c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.
Product Categories/Family for anti-c-erbB2, p185 antibody
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Product Notes
The c-erbB2, p185 (Catalog #AAA618482) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB2, p185 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Dilution: Immunoprecipitation: 10ug/mg protein lysate. Use Protein A. Western Blot: 5-10ug/ml for 2 hours at RT Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the c-erbB2, p185 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB2, p185, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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