Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with MBS618482 using peroxidase labeled and AEC chromogen. Note membrane staining of tumor cells.)
Rabbit c-erbB2 Polyclonal Antibody | anti-c-erbB2 antibody
c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free)
Reactivity
Human, Monkey, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
c-erbB2; Polyclonal Antibody; p185 (HER2; neu Receptor) (BSA & Azide Free); anti-c-erbB2 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Species Crossreactivity: monkey, rat and mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-c-erbB2 antibody
Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA)
Application Notes
IHC-P: 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
IP: 10ug/mg protein lysate. Use Protein A.
WB: 5-10ug/ml for 2 hours at RT
Applications are based on unconjugated antibody.
IP: 10ug/mg protein lysate. Use Protein A.
WB: 5-10ug/ml for 2 hours at RT
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide derived from the C-terminus of human c-erbB-2/HER-2 protein. Sequence is identical in rat neu protein. Cellular Localization: Cell membrane. Epitope: C-terminal.
Conjugate
MaxLight750
Positive Control
SKBR-3 cells, T47D cells or breast carcinomas.
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with MBS618482 using peroxidase labeled and AEC chromogen. Note membrane staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast cancer stained with MBS618482 using peroxidase labeled and AEC chromogen. Note membrane staining of tumor cells.)
Product Categories/Family for anti-c-erbB2 antibody
References
1. Gullick, W.J., et al., Int. J. Cancer 40(2): 246-254 (1987). 2. Gasparini, G., et al., Eur. J. Cancer 30A(1): 16-22 (1994). 3. Coombs, L.M., et al., J. Path. 169(1): 35-42 (1993). 4. Schlegel, J., et al., Acta Neuropathologica 86(5): 473-479 (1993). 5. Soomro, S., et al., Int. J. Cancer 55(1): 63-65 (1993). 6. Allred, D.C., et al., Human Path. 23(9): 974-979 (1992). 7. Allred, D.C., et al., J. Clin. Oncol. 10(4): 599-605 (1992). 8. Gasparini, G., et al., J. Clin. Oncol. 10(5): 686-695 (1992). 9. Hilton, D.A. & West, K.P., J. Clin. Path. 45(5): 454-456 (1992). 10. Hitchcock, A., et al., Am. J. Surg. Path. 16(1): 58-61 (1992). 11. Molina, R., et al., Anticancer Res. 12: 1965-1971 (1992). 12. Noguchi, M., et al., Cancer 69(12): 2953-2960 (1992). 13. Schroeter, C.A., et al., Br. J. Cancer 66(4): 728-734 (1992). 14. Allred, D.C., Tandon, A.K., Clark, G.M., McGuire, W.L.: HER-2/neu oncogene amplification and expression in human mammary carcinoma. Biochemical and Molecular Aspects of Selected Cancer, Vol. I (Chapter 3). Pretlow, T.G., Pretlow, T.P. (eds.), Academic Press 75-97 (1991). 15. Barnes, D.M., et al., Breast Cancer Res. and Treat. 18(1): 11-17 (1991). 16. Coombs, L.M., et al., Brit. J. Cancer 63(4): 601-608 (1991). 17. Hainsworth, P.J., et al., Eur. J. Surg. Oncol. 17(1): 9-15 (1991). 18. Lovekin, C., et al., [published erratum appears in Br. J. Cancer 64(1): 202 (1991)]. Br. J. Cancer 63(3): 439-443 (1991). 19. McCann, A.H., et al., Cancer Res. 51(12): 3296-3303 (1991). 20. Soomro, S., et al., J. Clin. Path. 44(3): 211-214 (1991). 21. Winstanley, J., et al., Br. J. Cancer 63(3): 447-450 (1991). 22. Bartkova, J., et al., Human Path. 21(11): 1164-1167 (1990). 23. Borg, A., et al., Cancer Res. 50: 4332-4337 (1990). 24. Haldane, J.S., et al., J. Path. 162(3): 231-237 (1990). 25. Hall, P.A., et al., J. Path. 161(3): 195-200 (1990). 26. McCann, A., et al., Cancer 65(1): 88-92 (1990). 27. Ramachandra, S., et al., J. Path. 161(1): 7-14 (1990). 28. Richner, J., et al., Ann. Oncol. 1(4): 263-268 (1990). 29. Gullick, W.J., et al., Int. J. Cancer 40(2): 246-54 (1987, Aug 15).
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Product Notes
The c-erbB2 (Catalog #AAA6466281) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA). IHC-P: 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. IP: 10ug/mg protein lysate. Use Protein A. WB: 5-10ug/ml for 2 hours at RT Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the c-erbB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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