Rabbit anti-Mouse, Rat BKCa Channel Polyclonal Antibody

BKCa Channel (High-conductance Ca2+ activated Potassium Channel, MaxiK Slo, KCNMA1)

Cat. Num. AAA618363

• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: BKCa Channel; Polyclonal Antibody; BKCa Channel (High-conductance Ca2+ activated Potassium Channel; MaxiK Slo; KCNMA1); Anti -BKCa Channel (High-conductance Ca2+ activated Potassium Channel; anti-BKCa Channel antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes BKCa Channel. Splice forms sequence homology
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.025% sodium azide, 40% glycerol.

• Applicable Applications for anti-BKCa Channel antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:200-1:500 (rat brain membranes); Preadsorption Control: 3ug fusion protein (B2103A) per 1ug antibody (B2103).; Immunohistochemistry: Rat uterus and brain sections.
• Immunogen: GST fusion protein corresponding to residues 1097-1196 of mouse Slo (mSlo) (Accession A48206).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blotting of non-differentiated PC12 cells lysate (30ug) 1. Anti-P2X2 antibody (1:200) 2. Anti-P2X2 antibody, preincubated.)

Testing Data

(Staining of the interpeduncular nucleus (IPN) in rat brain. Photo courtesy of Dr. Shai Shoham, Ph.D., Herzog...)

Related Product Information for anti-BKCa Channel antibody

Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

Product Categories/Family for anti-BKCa Channel antibody

Antibodies; Abs to Ion Channel