Mouse MAFF Monoclonal Antibody | anti-MAFF antibody

MAFF (V-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F (avian), U-MAF) (FITC)

Cat. Num. AAA6179019

• Gene Names: MAFF; U-MAF; hMafF
• Applications: Western Blot
• Purity: Purified
• Synonyms: MAFF; Monoclonal Antibody; MAFF (V-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F (avian); U-MAF) (FITC); V-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog F (avian); U-MAF; anti-MAFF antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1F8
• Specificity: Recognizes MAFF.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).

• Applicable Applications for anti-MAFF antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: MAFF (NP_036455.1, 1aa-110aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGK
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.; ; FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged MAFF is 0.03 ng/ml as a capture antibody.)

Western Blot (WB)

(MAFF monoclonal antibody (M02), clone 1F8. Western Blot analysis of MAFF expression in Hela S3 NE.)

Related Product Information for anti-MAFF antibody

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Product Categories/Family for anti-MAFF antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 6912490
• NCBI GeneID: 23764
• NCBI Accession #: NP_036455.1
• NCBI GenBank Nucleotide #: NM_012323.4
• UniProt Accession #: Q9ULX9; Q9Y525; B4DV49
• Molecular Weight: 14,569 Da
• NCBI Official Full Name: transcription factor MafF isoform a
• NCBI Official Synonym Full Names: MAF bZIP transcription factor F
• NCBI Official Symbol: MAFF
• NCBI Official Synonym Symbols: U-MAF; hMafF
• NCBI Protein Information: transcription factor MafF
• UniProt Protein Name: Transcription factor MafF
• Protein Family: MaFF-interacting protein
• UniProt Gene Name: MAFF
• UniProt Entry Name: MAFF_HUMAN

NCBI Description

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

MAFF: Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; in utero embryonic development; regulation of epidermal cell differentiation; parturition; blood coagulation