Goat anti-Human MUCDHL Polyclonal Antibody | anti-CDHR5 antibody
MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin)
Gene Names
CDHR5; MUCDHL; MUPCDH; MU-PCDH; FLJ20219; CDHR5
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by human MUCDHL affinity chromatography.
Synonyms
MUCDHL; Polyclonal Antibody; MUCDHL (Mucin and Cadherin-Like Protein; mu-Protocadherin); Anti -MUCDHL (Mucin and Cadherin-Like Protein; anti-CDHR5 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Selected for its ability to recognize human MUCDHL in direct ELISAs and Western Blots. In these formats, this antibody shows ~10% crossreactivity with rmMUCDHL.
Purity/Purification
Affinity Purified
Purified by human MUCDHL affinity chromatography.
Purified by human MUCDHL affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Applicable Applications for anti-CDHR5 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MUCDHL. The detection limit for rhMUCDHL is approximately 5 ng/lane under non-reducing and reducing conditions., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MUCDHL. The detection limit for rhMUCDHL is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
E. coli-derived rhMUCDHL (aa26-434)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CDHR5 antibody
Mucin and cadherin-like protein (MUCDHL), also known as -protocadherin, is a 110kD type I transmembrane protein that is both O- and N-glycosylated. It is a calcium-dependent cell adhesion protein in the cadherin superfamily. MUCDHL is found on intestinal and renal epithelium. Human MUCDHL extracellular domain (ECD) contains four cadherin domains (residues 71-459) plus several mucin domains (residues 540-645). Alternate splicing creates four isoforms that differ in their mucin domains. In the region used as immunogen, human and mouse MUCDHL share 63% amino acid sequence identity.
Product Categories/Family for anti-CDHR5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
69,610 Da
NCBI Official Full Name
MUCDHL
NCBI Official Synonym Full Names
cadherin-related family member 5
NCBI Official Symbol
CDHR5
NCBI Official Synonym Symbols
MUCDHL; MUPCDH; MU-PCDH; FLJ20219; CDHR5
NCBI Protein Information
cadherin-related family member 5; mu-protocadherin; OTTHUMP00000162884; mucin and cadherin-like protein
UniProt Protein Name
Mucin-like protocadherin
Protein Family
UniProt Gene Name
MUPCDH
UniProt Entry Name
Q58EZ6_HUMAN
NCBI Description
This gene is a novel mucin-like gene that is a member of the cadherin superfamily. While encoding nonpolymorphic tandem repeats rich in proline, serine and threonine similar to mucin proteins, the gene also contains sequence encoding calcium-binding motifs found in all cadherins. The role of the hybrid extracellular region and the specific function of this protein have not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
Uniprot Description
MUCDHL: Acts as a calcium-dependent cell adhesion protein. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein binding; beta-catenin binding; calcium ion binding
Biological Process: cell adhesion; homophilic cell adhesion
Research Articles on CDHR5
1. MUCDHL genes are biallelically expressed in multiple fetal and adult tissues both in humans and in mice
2. identifed the human mu-protocadherin ortholog and examined its expression in the developing kidney
2. identifed the human mu-protocadherin ortholog and examined its expression in the developing kidney
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Product Notes
The CDHR5 mupcdh (Catalog #AAA617598) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The MUCDHL (Mucin and Cadherin-Like Protein, mu-Protocadherin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUCDHL can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MUCDHL. The detection limit for rhMUCDHL is approximately 5 ng/lane under non-reducing and reducing conditions., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MUCDHL. The detection limit for rhMUCDHL is approximately 0.3 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the CDHR5 mupcdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUCDHL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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