Sheep anti-Human Tamm-Horsfall Glycoprotein Polyclonal Antibody

Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) (BSA, Azide & Glycerol free)

Cat. Num. AAA617579

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Gel Super Shift Assay
• Purity: Purified; Purified.
• Synonyms: Tamm-Horsfall Glycoprotein; Polyclonal Antibody; Tamm-Horsfall Glycoprotein (Uromucoid; THgp; THP; Uromodulin; UMOD) (BSA; Azide & Glycerol free); Anti -Tamm-Horsfall Glycoprotein (Uromucoid; anti-Tamm-Horsfall Glycoprotein antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Tamm-Horsfall Glycoprotein. One arc by 2D-IEP against Tamm-Horsfall glycoprotein (1mg/ml). No precipitin line by IEP against normal human plasma. Identity verified by double immunodiffusion.
• Purity/Purification: Purified; Purified.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Glycerol free.

• Applicable Applications for anti-Tamm-Horsfall Glycoprotein antibody: ELISA (EL/EIA), Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)
• Application Notes: Dilution: IEP: 100ul antiserum vs. 5ul uromucoid (50mg/L); RID and Rocket RID: 10ul antiserum/cm2 in gel vs. 5ul uromucoid (50mg/L), NT-1/5; Double Diffusion: 10ul antiserum vs. 3ul uromucoid (50mg/L); Note: The use of 3% PEG 6000, 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended for the above applications.
• Immunogen: Highly purified human Tamm-Horsfall glycoprotein (uromucoid) from urine. (SDS-PAGE > 95%).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Tamm-Horsfall Glycoprotein antibody

Tamm-Horsfall glycoprotein (THP), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that THP may act as a defense factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because THP-related proteins are also found in the superficial layers of the oral mucosa, THP may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that THP binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of THP with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of THP is crucial for its binding to PTX. The THP-PTX interaction was exploited to develop an affinity chromatography method that allows a one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.

Product Categories/Family for anti-Tamm-Horsfall Glycoprotein antibody

Antibodies; Abs to Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 2119691
• NCBI Official Full Name: Tamm-Horsfall protein - rat