Rabbit Glucosidase II, alpha Polyclonal Antibody | anti-GANAB antibody
Glucosidase II, alpha
Gene Names
GANAB; G2AN; GLUII; KIAA0088
Reactivity
Bovine, Canine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Applications
Western Blot, Immunoprecipitation
Purity
SerumSerum
Synonyms
Glucosidase II; alpha; Polyclonal Antibody; Anti -Glucosidase II; anti-GANAB antibody
Host
Rabbit
Reactivity
Bovine, Canine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects a ~110kD protein, corresponding to the apparent molecular mass of alpha-glucosidase II on SDS-PAGE. Species Crossreactivity: Human, mouse, rat, bovine, canine, hamster, monkey, porcine, rabbit and sheep.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, whole serum, before the addition of glycerol to 40%.
Applicable Applications for anti-GANAB antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Immunoprecipitation: 1:25
Western Blot (Colorimetric): 1:250; Note: It is recommended for Western Blot applications, that the secondary antibody be quenched by adding normal sera (of an identical species to secondary host) to the diluent.
Optimal dilutions to be determined by researcher.
Dilution: Immunoprecipitation: 1:25
Western Blot (Colorimetric): 1:250; Note: It is recommended for Western Blot applications, that the secondary antibody be quenched by adding normal sera (of an identical species to secondary host) to the diluent.
Optimal dilutions to be determined by researcher.
Immunogen
Human alpha-glucosidase II synthetic peptide, corresponding to aa916-930, prepared as a multiple antigen peptide (MAP).
Positive Control
Rat Brain Tissue Extract
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-GANAB antibody
alpha-Glucosidase II is a 110kD glycosylated endoplasmic reticulum resident protein that is involved in trimming of glucose from newly synthesized glycosylated proteins in the endoplasmic reticulum (ER). Together with an 80kD beta subunit, these polypeptides remove two alpha-1,3-linked glucose from high mannose oligosaccharides linked to asparagine residue on glycoprotein. alpha-Glucosidase II mediated glucose trimming is necessary for the interaction of substrates such as influenza virus hemagglutinin (HA), MHC Class I, and T-cell antigen receptor (TCR) with calnexin, an ER resident chaperone that is specific for monoglucosylated glycoproteins.
Product Categories/Family for anti-GANAB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
106,874 Da
NCBI Official Full Name
Glucosidase II
NCBI Official Synonym Full Names
glucosidase, alpha; neutral AB
NCBI Official Symbol
GANAB
NCBI Official Synonym Symbols
G2AN; GLUII; KIAA0088
NCBI Protein Information
neutral alpha-glucosidase AB; OTTHUMP00000236516; OTTHUMP00000236517; OTTHUMP00000236518; alpha-glucosidase 2; glucosidase II subunit alpha; alpha glucosidase II alpha subunit
UniProt Protein Name
Neutral alpha-glucosidase AB
Protein Family
UniProt Gene Name
GANAB
UniProt Synonym Gene Names
G2AN; KIAA0088
UniProt Entry Name
GANAB_HUMAN
Uniprot Description
GANAB: Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Belongs to the glycosyl hydrolase 31 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Glycan Metabolism - N-glycan biosynthesis; EC 3.2.1.84; Hydrolase; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: alpha-glucosidase II complex; Golgi apparatus; membrane; endoplasmic reticulum lumen; melanosome
Molecular Function: glucan 1,3-alpha-glucosidase activity; carbohydrate binding
Biological Process: cellular protein metabolic process; protein folding; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on GANAB
Similar Products
Product Notes
The GANAB ganab (Catalog #AAA617142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Glucosidase II, alpha reacts with Bovine, Canine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Glucosidase II, alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Immunoprecipitation and Western Blot. Dilution: Immunoprecipitation: 1:25 Western Blot (Colorimetric): 1:250; Note: It is recommended for Western Blot applications, that the secondary antibody be quenched by adding normal sera (of an identical species to secondary host) to the diluent. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the GANAB ganab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucosidase II, alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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