Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-5: Hela£¬A431£¬Hepg-2£¬293T and Raji cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 15 seconds)
Rabbit anti-Human GANAB Polyclonal Antibody | anti-GANAB antibody
GANAB Antibody
Gene Names
GANAB; G2AN; GIIA; GLUII
Reactivity
Human
Applications
Western Blot
Purity
Antigen Affinity Purification
Synonyms
GANAB; Polyclonal Antibody; GANAB Antibody; G2AN; GLUII; anti-GANAB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total GANAB protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.3 mg/ml (varies by lot)
Sequence Length
852
Applicable Applications for anti-GANAB antibody
Western Blot (WB)
Application Notes
Western Blot: 1:200-1:1000
Immunogen
Synthetic peptide of human GANAB
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-5: Hela£¬A431£¬Hepg-2£¬293T and Raji cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 15 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane 1-5: Hela£¬A431£¬Hepg-2£¬293T and Raji cell Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 15 seconds)
Related Product Information for anti-GANAB antibody
Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains a and b subunits (Glucosidase IIa and Glucosidase IIb) which form a defined heterodimeric complex.Glucosidase IIa is the catalyitc core of the enzyme and can function independently of the b subunit. The sequence of Glucosidase IIb encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C-terminus.
Product Categories/Family for anti-GANAB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
5,673 Da
NCBI Official Full Name
neutral alpha-glucosidase AB isoform 4
NCBI Official Synonym Full Names
glucosidase II alpha subunit
NCBI Official Symbol
GANAB
NCBI Official Synonym Symbols
G2AN; GIIA; GLUII
NCBI Protein Information
neutral alpha-glucosidase AB
UniProt Protein Name
Neutral alpha-glucosidase AB
Protein Family
UniProt Gene Name
GANAB
UniProt Synonym Gene Names
G2AN; KIAA0088
UniProt Entry Name
GANAB_HUMAN
Uniprot Description
GANAB: Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Belongs to the glycosyl hydrolase 31 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.2.1.84; Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; Hydrolase
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: endoplasmic reticulum lumen; extracellular matrix; membrane
Biological Process: protein folding
Disease: Polycystic Kidney Disease 3
Similar Products
Product Notes
The GANAB ganab (Catalog #AAA9415709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GANAB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GANAB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:200-1:1000. Researchers should empirically determine the suitability of the GANAB ganab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GANAB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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