Rabbit anti-Human Hamartin Polyclonal Antibody | anti-TSC1 antibody

Hamartin (TSC1, Tuberous Sclerosis 1)

Cat. Num. AAA617029

• Gene Names: TSC1; LAM; TSC; KIAA0243; MGC86987
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hamartin; Polyclonal Antibody; Hamartin (TSC1; Tuberous Sclerosis 1); Anti -Hamartin (TSC1; anti-TSC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Tuberous Sclerosis 1 (TSC1).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-TSC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.,; Dilution: Immunohistochemistry (Formalin fixed paraffin): 2.5ug/ml; Western Blot: 1ug/ml
• Immunogen: Synthetic peptide (KLH-conjugated). Epitope: 15 amino acid peptide from the middle region of human TSC.1. Will recognize both isoforms of TSC1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(TSC1 (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human heart, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(TSC1 (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human skeletal muscle, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-TSC1 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 241666464
• NCBI GeneID: 7248
• NCBI Accession #: NP_001155899.1
• NCBI GenBank Nucleotide #: NM_001162427.1
• UniProt Accession #: Q0VAM5; Q32NF0; Q59IT9; Q86WV8; Q92574; Q96RT3; Q96RT4
• Molecular Weight: 41,124 Da
• NCBI Official Full Name: hamartin isoform 4
• NCBI Official Synonym Full Names: tuberous sclerosis 1
• NCBI Official Symbol: TSC1
• NCBI Official Synonym Symbols: LAM; TSC; KIAA0243; MGC86987
• NCBI Protein Information: hamartin; tumor suppressor; OTTHUMP00000022439; tuberous sclerosis 1 protein
• UniProt Protein Name: Tuberous sclerosis 1
• Protein Family: Hamartin
• UniProt Gene Name: TSC1
• UniProt Entry Name: Q0VAM5_HUMAN

NCBI Description

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; growth cone; protein complex; membrane; intracellular membrane-bound organelle; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; GTPase activating protein binding; protein N-terminus binding; GTPase regulator activity

Biological Process: myelination; protein heterooligomerization; regulation of cell-matrix adhesion; regulation of cell cycle; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; cell projection organization and biogenesis; positive regulation of focal adhesion formation; synapse organization and biogenesis; kidney development; cell cycle arrest; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; glucose import; regulation of stress fiber formation; negative regulation of translation; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1