Western Blot (WB)
(Western Blot analysis of TFF1 expression in transfected 293T cell line by TFF1 monoclonal antibody. Lane 1: TFF1 transfected lysate (9.1kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human Trefoil Factor 1 Monoclonal Antibody | anti-TFF1 antibody
Trefoil Factor 1 (TFF1, Breast Cancer Estrogen-inducible Protein, BCEI, D21S21, HPS2, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, PS2) (PE)
Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Trefoil Factor 1; Monoclonal Antibody; Trefoil Factor 1 (TFF1; Breast Cancer Estrogen-inducible Protein; BCEI; D21S21; HPS2; PNR-2; Polypeptide P1.A; hP1.A; Protein pS2; PS2) (PE); anti-TFF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
M2
Specificity
Recognizes human TFF1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-TFF1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-85 from human TFF1 (AAH32811) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of TFF1 expression in transfected 293T cell line by TFF1 monoclonal antibody. Lane 1: TFF1 transfected lysate (9.1kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of TFF1 expression in transfected 293T cell line by TFF1 monoclonal antibody. Lane 1: TFF1 transfected lysate (9.1kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged TFF1 is ~0.1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged TFF1 is ~0.1ng/ml as a capture antibody.)
Related Product Information for anti-TFF1 antibody
The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair.
Product Categories/Family for anti-TFF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
9,150 Da
NCBI Official Full Name
Homo sapiens trefoil factor 1, mRNA
NCBI Official Synonym Full Names
trefoil factor 1
NCBI Official Symbol
TFF1
NCBI Official Synonym Symbols
pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
NCBI Protein Information
trefoil factor 1
Protein Family
NCBI Description
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
Research Articles on TFF1
Similar Products
Product Notes
The TFF1 (Catalog #AAA6160818) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Trefoil Factor 1 (TFF1, Breast Cancer Estrogen-inducible Protein, BCEI, D21S21, HPS2, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, PS2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Trefoil Factor 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TFF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trefoil Factor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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