Rabbit Smad1 Polyclonal Antibody | anti-Smp_013060 antibody

Smad1, phosphorylated (Ser463,465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)

Cat. Num. AAA615510

• Reactivity: Human, Mouse, Rat, Xenopus
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Smad1; Polyclonal Antibody; phosphorylated (Ser463; 465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -Smad1; anti-Smp_013060 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the dual serine phosphorylated human Smad1 (Ser 463/465), which in the phosphorylated state has an apparent molecular weight of approximately 60-65kD. Species Crossreactivity: Mouse (Predicted crossreactivity with rat and Xenopus).
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.2, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.

• Applicable Applications for anti-Smp_013060 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Immunoblot: 0.5-2ug/ml detected phosphorylated Smad1 in RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4. P19 cell lysate treated with BMP4 was resolved by electrophoresis, transferred to nitrocellulose and probed with phospho-Smad1 (Ser463/465) (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.; Note: A non-specific band was detected in P19 cell lysate, Mr 34-35kD.
• Immunogen: Synthetic peptide (CG-GSPHNPIS[pS]V[pS]-GG) corresponding to amino acids 455-465 of human Smad1 protein (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Smp_013060 antibody

The Drosophila gene Mothers against dpp (Mad) and the C. elegans sma genes are implicated in the signaling pathway downstream of Ser/Thr kinase receptors required for signaling by the TGFb superfamily. Phosphorylation of Smad1, a human homolog of MAD is regulated and rapidly induced by bone morphogenic proteins (BMP) but not TGFb or activin. BMPs play a role in ectopic bone formation and inductive interactions during the early development of both vertebrates and invertebrates. In the mouse, deletion of the BMP4 gene leads to mesoderm formation and early embryonic lethality. Phosphorylation of MADR1 in a BMP dependent manner is functionally important in that it results in accumulation of Smad1 in the nucleus, indicative of Smad and Smad-related molecules as essential components of the Ser/Thr kinase receptor signaling pathway.

Product Categories/Family for anti-Smp_013060 antibody

Antibodies; Abs to Smad Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11464653
• NCBI GeneID: 8342940
• UniProt Accession #: Q9GQN9
• Molecular Weight: 50,839 Da
• NCBI Official Full Name: Smad1
• NCBI Official Symbol: Smp_013060
• NCBI Protein Information: smad1 5 8 and
• UniProt Protein Name: Smad1
• UniProt Gene Name: Smad1
• UniProt Entry Name: Q9GQN9_SCHMA