Mouse anti-Human RPL11 Monoclonal Antibody | anti-RPL11 antibody

RPL11 (60S Ribosomal Protein L11, CLL-associated Antigen KW-12) (HRP)

Cat. Num. AAA6154721

• Gene Names: RPL11; L11; DBA7; GIG34
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL11; Monoclonal Antibody; RPL11 (60S Ribosomal Protein L11; CLL-associated Antigen KW-12) (HRP); anti-RPL11 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2A1
• Specificity: Recognizes human RPL11.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-RPL11 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to human RPL11, aa1-178 (AAH18970) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MADQGEKENPMRELRIRKLCLNICVGESGDRLTRAAKVLEQLTGQTPVFSKARYTVRSFGIRRNEKIAVHCTVRGAKAEEILEKGLKVREYELRKNNFSDTGNFGFGIQEHIDLGIKYDPSIGIYGLDFYVVLGRPGFSIADKKRRTGCIGAKHRISKEEAMRWFQQKYDGIILPGK
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-RPL11 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternative splice variants encoding different isoforms may exist, but they have not been fully characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].

Product Categories/Family for anti-RPL11 antibody

Antibodies; Ribosomal Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 40226081
• NCBI GeneID: 6135
• UniProt Accession #: P62913; P25121; P39026; Q8TDH2; Q9Y674
• Molecular Weight: 20,124 Da
• NCBI Official Full Name: Homo sapiens ribosomal protein L11, mRNA
• NCBI Official Synonym Full Names: ribosomal protein L11
• NCBI Official Symbol: RPL11
• NCBI Official Synonym Symbols: L11; DBA7; GIG34
• NCBI Protein Information: 60S ribosomal protein L11
• UniProt Protein Name: 60S ribosomal protein L11
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL11
• UniProt Entry Name: RL11_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L5P family of ribosomal proteins. It is located in the cytoplasm. The protein probably associates with the 5S rRNA. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Dec 2010]

Uniprot Description

RPL11: Binds to 5S ribosomal RNA. Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML. Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7). DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L5P family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Motility/polarity/chemotaxis; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: membrane; cytoplasm; nucleolus; cytosol

Molecular Function: rRNA binding; protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; ribosomal large subunit biogenesis and assembly; translational termination; viral infectious cycle; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; rRNA processing; protein targeting

Disease: Diamond-blackfan Anemia 7