Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phospholipase A-2-activating protein (PLAA) Standard Curve.)
Mouse Phospholipase A-2-Activating Protein (PLAA) ELISA Kit | PLAA elisa kit
Mouse Phospholipase A-2-Activating Protein (PLAA) ELISA Kit
Gene Names
Plaa; PLAP; Ufd3; PLA2P; AI536418; AU018445; AW208417; 2410007N06; D4Ertd618e
Reactivity
Mouse
Synonyms
Phospholipase A-2-Activating Protein (PLAA); Mouse Phospholipase A-2-Activating Protein (PLAA) ELISA Kit; PLAA; DOA1; FLJ11281; FLJ12699; PLA2P; PLAP; DOA1 homolog; phospholipase A2 activating protein; PLAA elisa kit
Reactivity
Mouse
Specificity
Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLAA. No significant cross-reactivity or interference between Mouse PLAA and analogues was observed.
Sequence Length
794
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phospholipase A-2-activating protein (PLAA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Phospholipase A-2-activating protein (PLAA) Standard Curve.)
Related Product Information for PLAA elisa kit
Description: This Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAA in samples. An antibody specific for PLAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phospholipase A2-activating protein (PLAP) is potentially important in regulating the inflammatory response through its activation of phospholipase A2, which catalyzes the release of arachidonic acid. By screening a human monocyte cDNA library with a mouse Plap cDNA, Chopra et al. (1999) isolated human PLAP cDNAs. The 738-amino acid human PLAP protein predicted by the cDNA sequence has a molecular mass of 80,826 kD; however, immunoblot analysis using antibodies against PLAP detected a 72- to 74-kD protein in human monocyte cell lysates. In mouse macrophages, an antisense Plap oligonucleotide blocked cholera toxin-induced arachidonic acid release, indicating a role for PLAP in the regulation of phospholipase A2 activation.
Background: Phospholipase A2-activating protein (PLAP) is potentially important in regulating the inflammatory response through its activation of phospholipase A2, which catalyzes the release of arachidonic acid. By screening a human monocyte cDNA library with a mouse Plap cDNA, Chopra et al. (1999) isolated human PLAP cDNAs. The 738-amino acid human PLAP protein predicted by the cDNA sequence has a molecular mass of 80,826 kD; however, immunoblot analysis using antibodies against PLAP detected a 72- to 74-kD protein in human monocyte cell lysates. In mouse macrophages, an antisense Plap oligonucleotide blocked cholera toxin-induced arachidonic acid release, indicating a role for PLAP in the regulation of phospholipase A2 activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phospholipase A-2-activating protein
NCBI Official Synonym Full Names
phospholipase A2, activating protein
NCBI Official Symbol
Plaa
NCBI Official Synonym Symbols
PLAP; Ufd3; PLA2P; AI536418; AU018445; AW208417; 2410007N06; D4Ertd618e
NCBI Protein Information
phospholipase A-2-activating protein
UniProt Protein Name
Phospholipase A-2-activating protein
Protein Family
UniProt Gene Name
Plaa
UniProt Synonym Gene Names
Plap; PLA2P; PLAP
UniProt Entry Name
PLAP_MOUSE
Research Articles on PLAA
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Product Notes
The Mouse PLAA plaa (Catalog #AAA9717344) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717344 ELISA Kit recognizes Mouse PLAA. It is sometimes possible for the material contained within the vial of "Phospholipase A-2-Activating Protein (PLAA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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