Testing Data
Rabbit Flk-1 Receptor Tyrosine Kinase Polyclonal Antibody
Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Flk-1 Receptor Tyrosine Kinase; Polyclonal Antibody; Flk-1 Receptor Tyrosine Kinase (KDR; VEGFR2); Anti -Flk-1 Receptor Tyrosine Kinase (KDR; anti-Flk-1 Receptor Tyrosine Kinase antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Flk-1 (KDR, VEGFR2). Species Crossreactivity: Human, mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See F4565-08X.
Applicable Applications for anti-Flk-1 Receptor Tyrosine Kinase antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 5-10ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 1:50 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Dilution: Western Blot: 5-10ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 1:50 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Immunogen
Synthetic peptide from C-terminus of the precursor form of the mouse Flk-1. Cellular Localization: Cytoplasmic
Positive Control
Hey cells. Angiosarcoma
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human angiosarcoma stained with Flk-1/ VEGFR2 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human angiosarcoma stained with Flk-1/ VEGFR2 Ab using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.)
Related Product Information for anti-Flk-1 Receptor Tyrosine Kinase antibody
Flk-1/KDR/vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
Product Categories/Family for anti-Flk-1 Receptor Tyrosine Kinase antibody
Similar Products
Product Notes
The Flk-1 Receptor Tyrosine Kinase (Catalog #AAA615221) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Flk-1 Receptor Tyrosine Kinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 5-10ug/ml for 2hrs at RT. Immunohistochemistry (formalin/paraffin): 1:50 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the Flk-1 Receptor Tyrosine Kinase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Flk-1 Receptor Tyrosine Kinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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