Mouse anti-Human DLD Monoclonal Antibody | anti-DLD antibody

DLD (Dihydrolipoyl Dehydrogenase, Mitochondrial, Dihydrolipoamide Dehydrogenase, Glycine Cleavage System L Protein, GCSL, LAD, PHE3) (FITC)

Cat. Num. AAA6146854

• Gene Names: DLD; E3; LAD; DLDD; DLDH; GCSL; PHE3
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DLD; Monoclonal Antibody; DLD (Dihydrolipoyl Dehydrogenase; Mitochondrial; Dihydrolipoamide Dehydrogenase; Glycine Cleavage System L Protein; GCSL; LAD; PHE3) (FITC); anti-DLD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 3C1
• Specificity: Recognizes human DLD.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 2099

• Applicable Applications for anti-DLD antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-509 from human DLD (AAH18696) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MQSWSRVYCSLAKRGHFNRISHGLQGLSAVPLRTYADQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHMAHGKDFASRGIEMSEVRLNLDKMMEQKSTAVKALTGGIAHLFKQNKVVHVNGYGKITGKNQVTATKADGGTQVIDTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKMVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGVGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSIEAASGGKAEVITCDVLLVCIGRRPFTKNLGLEELGIELDPRGRIPVNTRFQTKIPNIYAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGIEYKVGKFPFAANSRAKTNADTDGMVKILGQKSTDRVLGAHILGPGAGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (81.73kD).)

Western Blot (WB)

(DLD monoclonal antibody, Western Blot analysis of DLD expression in Hela.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to DLD on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged DLD is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-DLD antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Quantitative Analysis of HIV-1 Infected CD4+ Cell Proteome: Dysregulated Cell Cycle Progression and Nuclear Transport Coincide with Robust Virus Production. Chan EY, Qian WJ, Diamond DL, Liu T, Gritsenko MA, Monroe ME, Camp DG 2nd, Smith RD, Katze MG.J Virol. 2007 Jul;81(14):7571-83. Epub 2007 May 9.

NCBI and Uniprot Product Information

• NCBI GI #: 33990946
• NCBI GeneID: 1738
• NCBI Official Full Name: Homo sapiens dihydrolipoamide dehydrogenase, mRNA
• NCBI Official Synonym Full Names: dihydrolipoamide dehydrogenase
• NCBI Official Symbol: DLD
• NCBI Official Synonym Symbols: E3; LAD; DLDD; DLDH; GCSL; PHE3
• NCBI Protein Information: dihydrolipoyl dehydrogenase, mitochondrial
• Protein Family: Delta-like protein

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]