Chicken anti-Canine, Rat Neuroglobin Polyclonal Antibody | anti-NGB antibody

Neuroglobin

Cat. Num. AAA614213

• Gene Names: NGB; NGB
• Reactivity: Canine, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Neuroglobin; Polyclonal Antibody; Anti -Neuroglobin; anti-NGB antibody

• Host: Chicken
• Reactivity: Canine, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human Neuroglobin at ~17kD.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with 50ul sterile ddH2O.
• Concentration: ~1mg/ml (after reconstitution) (varies by lot)
• Sequence: MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E

• Applicable Applications for anti-NGB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Other applications not tested.
• Immunogen: Recombinant protein corresponding to 150aa from human Neuroglobin.
• Control No.: C15031782
• Revision No.: 061813
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NGB antibody

Neuroglobin, a 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O2 supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucleotide and enhanced by neuroglobin overexpression.

Product Categories/Family for anti-NGB antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 10864065
• NCBI GeneID: 58157
• NCBI Accession #: NP_067080.1
• NCBI GenBank Nucleotide #: NM_021257.3
• UniProt Accession #: Q9NPG2
• Molecular Weight: 16,933 Da
• NCBI Official Full Name: neuroglobin
• NCBI Official Synonym Full Names: neuroglobin
• NCBI Official Symbol: NGB
• NCBI Official Synonym Symbols: NGB
• NCBI Protein Information: neuroglobin
• UniProt Protein Name: Neuroglobin
• Protein Family: Neuroglobin
• UniProt Gene Name: NGB
• UniProt Entry Name: NGB_HUMAN

NCBI Description

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq]

Uniprot Description

NGB: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Belongs to the globin family.

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: mitochondrion; perikaryon

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport; apoptosis