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Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.)

Rabbit HRas Polyclonal Antibody | anti-HRas antibody

HRas Antibody

Gene Names
HRAS; CTLO; KRAS; HAMSV; HRAS1; KRAS2; RASH1; RASK2; Ki-Ras; p21ras; C-H-RAS; c-K-ras; H-RASIDX; c-Ki-ras; C-BAS/HAS; C-HA-RAS1
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
HRas; Polyclonal Antibody; HRas Antibody; Affinity Purified Rabbit Polyclonal HRas Antibody; Polyclonal HRas antibody; Anti-HRas antibody; K-RAS antibody; RASH1 antibody; H-RASIDX antibody; HRAS1 antibody; C-HA-RAS1 antibody; Ha-Ras1 proto-oncoprotein antibody; CTLO antibody; N-RAS antibody; C-BAS/HAS antibody; C-H-RAS antibody; HAMSV antibody; transforming protein p21 antibody; HRAS antibody; anti-HRas antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-HRas antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:200
ICC: 1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: Hela; B: Hep G2; C: Molt-4) using a 12% SDS PAGE gel and HRas antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: Hela; B: Hep G2; C: Molt-4) using a 12% SDS PAGE gel and HRas antibody at a dilution of 1:1000)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded OVCAR3 xenograft using HRas antibody at a dilution of 1:500)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded OVCAR3 xenograft using HRas antibody at a dilution of 1:500)
Related Product Information for anti-HRas antibody
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities.
Product Categories/Family for anti-HRas antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
21 kDa
NCBI Official Full Name
HRAS, partial
NCBI Official Synonym Full Names
HRas proto-oncogene, GTPase
NCBI Official Symbol
HRAS
NCBI Official Synonym Symbols
CTLO; KRAS; HAMSV; HRAS1; KRAS2; RASH1; RASK2; Ki-Ras; p21ras; C-H-RAS; c-K-ras; H-RASIDX; c-Ki-ras; C-BAS/HAS; C-HA-RAS1
NCBI Protein Information
GTPase HRas
Protein Family

NCBI Description

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, cognitive disability, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]

Research Articles on HRas

Similar Products

Product Notes

The HRas (Catalog #AAA5312496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HRas can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:200 ICC: 1:100-1:200 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the HRas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRas, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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