Rabbit JAK1 Polyclonal Antibody | anti-jak1 antibody

JAK1 (Janus Kinase 1)

Cat. Num. AAA613520

• Gene Names: jak1; jak1
• Reactivity: Hamster, Human, Mouse, Rabbit, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Serum; Whole rabbit antiserum
• Synonyms: JAK1; Polyclonal Antibody; JAK1 (Janus Kinase 1); Anti -JAK1 (Janus Kinase 1); anti-jak1 antibody

• Host: Rabbit
• Reactivity: Hamster, Human, Mouse, Rabbit, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JAK1 (~130kD) and a degradation product of JAK1 (~110kD). Species Crossreactivity: mouse, rat, rabbit and Chinese hamster
• Purity/Purification: Serum; Whole rabbit antiserum
• Form/Format: Supplied as a liquid, 0.02% sodium azide.

• Applicable Applications for anti-jak1 antibody: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
• Application Notes: Immunoprecipitation: 5ul per 1-2mg of RIPA cell lysate. Tested using a RIPA lysate from mouse CTLL lymphocytes, TF-1 erythroleukemic cells and mouse thymus. Note: The amount of JAK1 in cell lines tends to be extremely low. It is highly recommended that a RIPA cell lysate (1-2mg protein) be prepared. JAK1 should be ?rst concentrated by Immunoprecipitation (5ul JAK1 antiserum) and then Western Blot (1:500 dilution). Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic HPLC-purified (>98%) peptide corresponding to 21aa (KTLIEKERFYESRCRPVTPS-C) of human JAK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-jak1 antibody

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Product Categories/Family for anti-jak1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 226237498
• NCBI GeneID: 30280
• UniProt Accession #: O12990; O73880; Q7ZU16
• Molecular Weight: 132,481 Da
• NCBI Official Full Name: jak1
• NCBI Official Synonym Full Names: Janus kinase 1
• NCBI Official Symbol: jak1
• NCBI Official Synonym Symbols: jak1
• NCBI Protein Information: tyrosine-protein kinase JAK1; jak-1; jak1 kinase; etID309794.6
• UniProt Protein Name: Tyrosine-protein kinase JAK1
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: jak1
• UniProt Entry Name: JAK1_DANRE

Uniprot Description

Function: Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location: Endomembrane system; Peripheral membrane protein

By similarity. Note: Wholly intracellular, possibly membrane associated

Developmental stage: Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1

Domain: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.Contains 1 FERM domain.Contains 1 protein kinase domain.Contains 1 SH2 domain.