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Mouse anti-Human AP2S1 Monoclonal Antibody | anti-AP2S1 antibody
AP2S1 (Adaptor-related Protein Complex 2 sigma 1 Subunit, AP2 Complex Subunit sigma 1, AP-2 Complex Subunit sigma 1, AP 17, AP17, AP17 delta, Clathrin Assembly Protein 2 Small Chain, Clathrin-associated/assembly/adaptor Protein Small 2, CLAPS2, Clathrin C
Gene Names
AP2S1; AP17; FBH3; HHC3; FBHOk; CLAPS2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AP2S1; Monoclonal Antibody; AP2S1 (Adaptor-related Protein Complex 2 sigma 1 Subunit; AP2 Complex Subunit sigma 1; AP-2 Complex Subunit sigma 1; AP 17; AP17; AP17 delta; Clathrin Assembly Protein 2 Small Chain; Clathrin-associated/assembly/adaptor Protein Small 2; CLAPS2; Clathrin C; anti-AP2S1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3E4
Specificity
Recognizes human AP2S1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-AP2S1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-142 from human AP2S1 (AAH06337) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (41.36kD).)
Western Blot (WB)
(Western Blot analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody. Lane 1: AP2S1 transfected lysate (17kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western blot analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with AP2S1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Testing Data
(Detection limit for recombinant GST tagged AP2S1 is 0.3ng/ml as a capture antibody.)
Related Product Information for anti-AP2S1 antibody
Adapter-related protein complex 2 sigma-1 subunit (AP2S1, AP17) is the component of the adaptor complexes which links clathrin to receptors in coated vesicles.It is the smallest polypeptide chain component of AP-2, a heterotetramer composed of two large adaptins (alpha-type subunit, AP2 alpha, and beta-type subunit, AP2 beta), a medium adaptin (mu-type subunit, AP2 mu1) and a small adaptin (sigma-type subunit, AP2S1).AP-2 is one of two major clathrin-associated protein complexes that interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.
Product Categories/Family for anti-AP2S1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
12,417 Da
NCBI Official Full Name
Homo sapiens adaptor-related protein complex 2, sigma 1 subunit, mRNA
NCBI Official Synonym Full Names
adaptor related protein complex 2 sigma 1 subunit
NCBI Official Symbol
AP2S1
NCBI Official Synonym Symbols
AP17; FBH3; HHC3; FBHOk; CLAPS2
NCBI Protein Information
AP-2 complex subunit sigma
Protein Family
NCBI Description
One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
