Rabbit anti-Human Cks1 Polyclonal Antibody | anti-CKS1B antibody

Cks1, CT (Cdc kinase subunit 1)

Cat. Num. AAA612642

• Gene Names: CKS1B; CKS1; ckshs1; PNAS-16; PNAS-18
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by epitope affinity chromatography.
• Synonyms: Cks1; Polyclonal Antibody; CT (Cdc kinase subunit 1); Anti -Cks1; anti-CKS1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Cks1 protein at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity: HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.
• Purity/Purification: Affinity Purified; Purified by epitope affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

• Applicable Applications for anti-CKS1B antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.; Dilution: ELISA: 0.1-1ug/ml; Western Blot: 1-3ug/ml; Immunofluorescence: 10ug/ml; Immunoprecipitation: 7ug/IP reaction; Immunohistochemistry: 2.5ug/ml; Note: For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, Heat Induced Epotope Retrieval (HIER) with citrate buffer, pH 6.0, is recommended.
• Immunogen: Synthetic peptide derived from the C-terminal region of human and mouse Cks1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CKS1B antibody

Cks (Cdc kinase subunit) proteins are members of the cyclin-dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets. Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells. Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase. Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27. High expression of Cks1 may be involved in the pathogenesis of human non-small cell lung carcinoma.

Product Categories/Family for anti-CKS1B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4502857
• NCBI GeneID: 1163
• NCBI Accession #: NP_001817.1
• NCBI GenBank Nucleotide #: NM_001826.2
• UniProt Accession #: P61024; P33551; Q5T178; Q9BZU3
• Molecular Weight: 9,660 Da
• NCBI Official Full Name: cyclin-dependent kinases regulatory subunit 1
• NCBI Official Synonym Full Names: CDC28 protein kinase regulatory subunit 1B
• NCBI Official Symbol: CKS1B
• NCBI Official Synonym Symbols: CKS1; ckshs1; PNAS-16; PNAS-18
• NCBI Protein Information: cyclin-dependent kinases regulatory subunit 1; CKS-1; PNAS-143; OTTHUMP00000035541; CDC28 protein kinase 1; CDC28 protein kinase 1B; CDC2-associated protein CKS1; cell division control protein CKS1; NB4 apoptosis/differentiation related protein
• UniProt Protein Name: Cyclin-dependent kinases regulatory subunit 1
• UniProt Gene Name: CKS1B
• UniProt Synonym Gene Names: CKS1
• UniProt Entry Name: CKS1_HUMAN

NCBI Description

CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS1B mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects a specialized role for the encoded protein. At least two transcript variants have been identified for this gene, and it appears that only one of them encodes a protein. [provided by RefSeq]

Uniprot Description

CKS1: Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. Belongs to the CKS family.

Protein type: Protein kinase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: nucleoplasm

Molecular Function: protein binding; cyclin-dependent protein kinase regulator activity

Biological Process: cell proliferation; cell division; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G1/S transition of mitotic cell cycle