Lambda Light Chain Secondary Antibody

Lambda Light Chain (APC)

Cat. Num. AAA6122310

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Lambda Light Chain; Monoclonal Secondary Antibody; Lambda Light Chain (APC); Lambda Light Chain secondary antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: LcN-2
• Specificity: Recognizes human lambda light chain. Useful in identifying leukemias, plasmacytomas, and some non-Hodgkin's lymphomas.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Allophycocyanin (APC).

• Applicable Applications for Lambda Light Chain secondary antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 0.5-1ug/500ug protein lysate; IF: 1-2ug/ml; FC/FACS: 0.5-1ug/10e6 cells; Western Blot: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Human IgG
• Conjugate: APC
• Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonsil stained with Lambda LcN-2 antibody (1:200), peroxidase-conjugate and DAB chromogen. Note specific cytoplasmic staining.)

Product Categories/Family for Lambda Light Chain secondary antibody

Antibodies; IgG Lambda

References

1. Campbell JP et. al. J Immunol Methods. 2013;391(1-2):1-13.

NCBI and Uniprot Product Information

• NCBI GI #: 126541
• NCBI GeneID: 3535
• UniProt Accession #: P01701; P01842
• Molecular Weight: ~22.5kDa
• NCBI Official Full Name: Ig lambda chain V-I region NEW
• UniProt Protein Name: Ig lambda chain V-I region NEW
• UniProt Entry Name: LV103_HUMAN

Uniprot Description

IGLV1-51: V segment of the variable region of immunoglobulins light chain that participates to the antigen recognition. Immunoglobulins (Igs), also known as antibodies, are membrane- bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors which, upon binding of a specific antigen (Ag), trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable region of one heavy chain, together with that of its associated light chain. Thus, each Ig has two antigen binding sites with remarkable affinity for a particular antigen. The variable regions are assembled by a process called V(D)J recombination and can then be subjected to somatic hypermutation after exposure to antigen to allow affinity maturation for a particular Ag (PubMed:20176268, PubMed:17576170). {ECO:0000303|PubMed:17576170, ECO:0000303|PubMed:20176268, ECO:0000303|PubMed:22158414}. Immunoglobulins are composed of two identical heavy chains and two identical light chains, linked by disulfide bonds. {ECO:0000303|PubMed:20176268}