Rabbit anti-Human, Rat Acid Sensing Ion Channels 4 Polyclonal Antibody | anti-ACCN4 antibody

Acid Sensing Ion Channels 4 (ASIC4)

Cat. Num. AAA611363

• Gene Names: ACCN4; ASIC4; BNAC4; MGC17248; MGC24860
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Acid Sensing Ion Channels 4; Polyclonal Antibody; Acid Sensing Ion Channels 4 (ASIC4); Anti -Acid Sensing Ion Channels 4 (ASIC4); anti-ACCN4 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Acid Sensing Ion Channels 4 (ASIC4). No significant sequence homology is detected with other ASIC-beta or other ASICs.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-ACCN4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1-10ug/ml (ECL); ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml with Control Peptide.
• Immunogen: Synthetic peptide 19aa sequence of human ASIC4 (KLH coupled). Location: ~N-terminal; Extracellular. Species Sequence Homology: Rat, mouse: 100%.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ACCN4 antibody

Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC); (3) mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N- and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride.

The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASICs for Acid Sensing Ion Channels). Three are at least 4 distinct proteins in ASIC family ASIC1-4. AISC4/SPASIC (human 539/666aa, chromosome 2; rat 539aa, ~97% homology between rat and human) is strongly expressed in pituitary and also detected in most areas of brain. ASIC4 is the most divergent member of ASIC family showing only 45% identity with ASIC1-3. ASIC4 is not activated by low pH. It may associate with other ASICs to form a functional channel.

Product Categories/Family for anti-ACCN4 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 256773230
• NCBI GeneID: 55515
• NCBI Accession #: NP_878267.2
• NCBI GenBank Nucleotide #: NM_182847.2
• UniProt Accession #: Q96FT7; Q53SB7; Q6GMS1; Q6PIN9; Q9NQA4
• Molecular Weight: 70,105 Da
• NCBI Official Full Name: amiloride-sensitive cation channel 4 isoform 2
• NCBI Official Synonym Full Names: amiloride-sensitive cation channel 4, pituitary
• NCBI Official Symbol: ACCN4
• NCBI Official Synonym Symbols: ASIC4; BNAC4; MGC17248; MGC24860
• NCBI Protein Information: amiloride-sensitive cation channel 4; OTTHUMP00000064871; OTTHUMP00000064872; brain sodium channel 4; acid-sensing ion channel 4
• UniProt Protein Name: Amiloride-sensitive cation channel 4
• UniProt Gene Name: ACCN4
• UniProt Synonym Gene Names: ASIC4
• UniProt Entry Name: ACCN4_HUMAN

NCBI Description

This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. Alternative splicing at this locus results in two transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

ASIC4: Probable cation channel with high affinity for sodium. In vitro, has no proton-gated channel activity. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC4 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral; Channel, sodium; Transporter

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: integral to plasma membrane

Molecular Function: sodium ion transmembrane transporter activity; ion channel activity; sodium channel activity

Biological Process: transport