Rabbit anti-Human ZO-1 Polyclonal Antibody | anti-Tjp1 antibody

ZO-1 (Mid) (Tight Junction-associated Polypeptide, Zonula Occludens 1)

Cat. Num. AAA611134

• Gene Names: Tjp1; ZO1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ZO-1; Polyclonal Antibody; ZO-1 (Mid) (Tight Junction-associated Polypeptide; Zonula Occludens 1); Anti -ZO-1 (Mid) (Tight Junction-associated Polypeptide; anti-Tjp1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ZO-1 protein at ~225kD. Bands of unknown origin may be observed at lower molecular weights in some sample lysates. These bands may represent breakdown products.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Tjp1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: Western Blot: 1-3ug/ml; Immunofluorescence: 2.5ug/ml; Immunohistochemistry (Frozen): 2ug/ml with frozen mouse liver, blood vessel (heart) and mossy fiber terminal (brain) tissues
• Immunogen: Synthetic peptide corresponding to the mid region of human ZO-1, which differs from canine, mouse, and rat by one conservative amino acid replacement.
• Positive Control: Canine MDCK-II, human A431, Caco-2, rat Rat-1 and NRK-52E cell lysates by WB.; MDCK-II, Caco-2, Rat-1 and NRK-52E cells by IF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Tjp1 antibody

The tight junction is a cell-to-cell adhesion structure in epithelial cells that constitute the epithelial junctional complex with adherens junctions and desmosomes. Tight junctions seal cells to create a primary barrier to the diffusion of solutes across the cellular sheet and also function as a boundary between the apical and basolateral membrane domains to produce their polarization.1 Tight junction strands are mainly composed of claudins, occludin, and JAM.2 Various scaffold proteins have been reported to be concentrated at the cytoplasmic surfaces of the junctional complex regions to determine the specialization and localization of junctions: ZO-1, ZO-2, and ZO-3. The zona occludins (ZO) proteins constitute the plaque structures underlying plasma membranes together with various proteins including cingulin, symplekin, Par-3·Par-6·atypical protein kinase C complex, ZONAB, and guanine nucleotide exchange factor-H1/Lfc.2 All three ZO proteins have three PDZ domains, one Src homology 3 domain, and one guanylate kinase-like homologue domain in this order from their NH2 termini, indicating that ZO-1, ZO-2, and ZO-3 are membrane-associated guanylate kinase-like homologues (MAGUKs).

Product Categories/Family for anti-Tjp1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 303710
• NCBI GeneID: 21872
• UniProt Accession #: P39447; E9QK00; Q91YW2
• Molecular Weight: 194,742 Da
• NCBI Official Full Name: ZO-1
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: Tjp1
• NCBI Official Synonym Symbols: ZO1
• NCBI Protein Information: tight junction protein ZO-1; OTTMUSP00000017707; zona occludens protein 1; zonula occludens protein 1
• UniProt Protein Name: Tight junction protein ZO-1
• Protein Family: Tight junction protein
• UniProt Gene Name: Tjp1
• UniProt Synonym Gene Names: Zo1
• UniProt Entry Name: ZO1_MOUSE

Uniprot Description

ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: apical junction complex; intercellular canaliculus; tight junction; cell surface; basolateral plasma membrane; apicolateral plasma membrane; intercellular junction; adherens junction; cell-cell adherens junction; membrane; apical part of cell; apical plasma membrane; cytoplasm; gap junction; plasma membrane; nucleus; cell junction

Molecular Function: protein C-terminus binding; calmodulin binding; protein domain specific binding; protein binding

Biological Process: sensory perception of sound; blastocyst formation