Flow Cytometry (FC/FACS)
(Flow Cytometry of human PBMCs, unstimulated (A) or activated with PMA/Ca2+ ionomycin/LPS and recombinant human IL-23 (B), were stained with CD3 epsilon (PE) and MBS609464. Quadrant markers were set based upon isotype control staining.)
Mouse anti-Human SIGIRR Monoclonal Antibody | anti-SIGIRR antibody
SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobulin Domain-containing IL1R-related Protein, Toll/Interleukin-1 Receptor 8, TIR8, UNQ301/PRO342) (APC)
Gene Names
SIGIRR; TIR8; MGC110992
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
SIGIRR; Monoclonal Antibody; SIGIRR (Single Ig IL-1-related Receptor; Single Ig IL-1R-related Molecule; Single Immunoglobulin Domain-containing IL1R-related Protein; Toll/Interleukin-1 Receptor 8; TIR8; UNQ301/PRO342) (APC); Anti -SIGIRR (Single Ig IL-1-related Receptor; anti-SIGIRR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10K61
Specificity
Recognizes human SIGIRR.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-SIGIRR antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using human PBMCs.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of S1013-68G to 1-2.5 x 10e5 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using human PBMCs.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of S1013-68G to 1-2.5 x 10e5 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody.
Immunogen
Recombinant corresponding to human SIGIRR expressed in NSO cells (Q6IA17).
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing Allophycocyanin conjugates will result in a substantial loss of enzymatic activity.
Flow Cytometry (FC/FACS)
(Flow Cytometry of human PBMCs, unstimulated (A) or activated with PMA/Ca2+ ionomycin/LPS and recombinant human IL-23 (B), were stained with CD3 epsilon (PE) and MBS609464. Quadrant markers were set based upon isotype control staining.)
Flow Cytometry (FC/FACS)
(Flow Cytometry of human PBMCs, unstimulated (A) or activated with PMA/Ca2+ ionomycin/LPS and recombinant human IL-23 (B), were stained with CD3 epsilon (PE) and MBS609464. Quadrant markers were set based upon isotype control staining.)
Related Product Information for anti-SIGIRR antibody
SIGIRR is a type I transmembrane protein belonging to the IL-1 receptor superfamily. It has a single extracellular Ig-like domain and conserved motifs in its cytoplasmic domain that are characteristic of the IL-1 receptor and Toll-like receptor families.
Product Categories/Family for anti-SIGIRR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,679 Da
NCBI Official Full Name
single Ig IL-1-related receptor
NCBI Official Synonym Full Names
single immunoglobulin and toll-interleukin 1 receptor (TIR) domain
NCBI Official Symbol
SIGIRR
NCBI Official Synonym Symbols
TIR8; MGC110992
NCBI Protein Information
single Ig IL-1-related receptor; OTTHUMP00000229641; OTTHUMP00000229642; OTTHUMP00000229643; OTTHUMP00000229644; OTTHUMP00000229645; toll/interleukin-1 receptor 8; single Ig IL-1R-related molecule; single immunoglobulin domain IL1R1 related; single immunoglobulin domain-containing IL1R-related protein
UniProt Protein Name
Single Ig IL-1-related receptor
Protein Family
UniProt Gene Name
SIGIRR
UniProt Entry Name
SIGIR_HUMAN
Uniprot Description
SIGIRR: Acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. Attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Through its extracellular domain interferes with the heterodimerization of Il1R1 and IL1RAP. Belongs to the interleukin-1 receptor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: membrane; integral to membrane
Molecular Function: protein binding
Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of transcription factor activity; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; signal transduction; negative regulation of chemokine biosynthetic process
Research Articles on SIGIRR
Similar Products
Product Notes
The SIGIRR sigirr (Catalog #AAA609464) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SIGIRR (Single Ig IL-1-related Receptor, Single Ig IL-1R-related Molecule, Single Immunoglobulin Domain-containing IL1R-related Protein, Toll/Interleukin-1 Receptor 8, TIR8, UNQ301/PRO342) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SIGIRR can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using human PBMCs. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of S1013-68G to 1-2.5 x 10e5 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody. Researchers should empirically determine the suitability of the SIGIRR sigirr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGIRR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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