Testing Data
(Whole blood monocytes were stained with MBS606914 (filled histogram) or isotype control (open histogram).)
Rat anti-Human Tim3 Monoclonal Antibody | anti-HAVCR2 antibody
Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC)
Gene Names
HAVCR2; TIM3; KIM-3; TIMD3; Tim-3; FLJ14428; HAVCR2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Tim3; Monoclonal Antibody; Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3; T Cell Immunoglobulin Mucin 3; TIM3; TIM-3; TIMD3; FLJ14428; Hepatitis A Virus Cellular Receptor 2; HAVCR 2; HAVCR2; HAVCR-2; Kidney Injury Molecule 3; KIM-3) (APC); Anti -Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3; anti-HAVCR2 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2A
Clone Number
9L856
Specificity
Recognizes human Tim3.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC)
Concentration
0.01 mg/ml (varies by lot)
Applicable Applications for anti-HAVCR2 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated using whole blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of T5469-92F to up to 1x10e6 cells and incubate for 30 minutes at RT
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2A antibody.
Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated using whole blood monocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of T5469-92F to up to 1x10e6 cells and incubate for 30 minutes at RT
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2A antibody.
Immunogen
Recombinant protein corresponding to aa22-200 from the extracellular domain of human Tim3 expressed in NSO cells (NP_116171).
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.
Testing Data
(Whole blood monocytes were stained with MBS606914 (filled histogram) or isotype control (open histogram).)
Testing Data
(Whole blood monocytes were stained with MBS606914 (filled histogram) or isotype control (open histogram).)
Related Product Information for anti-HAVCR2 antibody
T cell immunoglobulin mucin-3 (Tim3) is a member of the T cell Immunoglobulin- and Mucin-domain-containing family of type I membrane glycoproteins that regulate autoimmune and allergic disease. Tim3 is selectively expressed on Th1 cells and interacts with galectin-9. It negatively regulates Th1 responses and affects macrophage activation. The 280aa mature human Tim3 contains a V-type Ig-like domain that shows multiple polymorphisms, followed by a mucin-like domain in the 171aa extracellular region, which shares 60% aa identity with the mouse Tim3 ECD. One splice variant is truncated within the mucin domain and presumably is secreted.
Product Categories/Family for anti-HAVCR2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,394 Da
NCBI Official Full Name
T cell immunoglobulin mucin-3
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
HAVCR2
NCBI Official Synonym Symbols
TIM3; KIM-3; TIMD3; Tim-3; FLJ14428; HAVCR2
NCBI Protein Information
hepatitis A virus cellular receptor 2; TIMD-3; HAVcr-2; kidney injury molecule-3; T-cell membrane protein 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3
UniProt Protein Name
Hepatitis A virus cellular receptor 2
Protein Family
UniProt Gene Name
HAVCR2
UniProt Synonym Gene Names
TIM3; TIMD3
UniProt Entry Name
HAVR2_HUMAN
NCBI Description
CD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.[supplied by OMIM]
Uniprot Description
TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5q33.3
Cellular Component: integral to membrane
Disease: Rheumatoid Arthritis
Research Articles on HAVCR2
1. expression is increased on Vdelta T cells of pregnant women
2. Mucin domain-containing molecule 3 (Tim-3) is preferentially expressed in lymphoma-derived endothelial cells.
3. Structural and biologic studies reveal that TIM-3 is a receptor for phosphatidylserine (PtdSer) and that TIM-3 polymorphic variants differ functionally in their recognition of PtdSer and clearance of apoptotic cells.
4. The TIM-3 pathway and the therapeutic potential of modulating the pathway in systemic lupus erythematosus, is reviewed.
5. haplotypes including TIM-3 genetic variants confer susceptibility to asthma in a Chinese population
6. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
7. Tim-3 expression correlates with a dysfunctional and senescent phenotype (CD127(low) CD57(high)), a central rather than effector memory profile (CD45RA(negative) CCR7(high)), and reduced Th1/Tc1 cytokine production.
8. These results suggest that TIM-3 is a negative regulator of human T cells and regulates Th1 and Th17 cytokine secretion.
9. Reduced TIM-3 expression in the lungs of patients may result in a defective T cell ability to control the Th1 immune response and could thus contribute to the pathogenesis of sarcoidosis.
10. The -1516G>T, -574T>G, and 4259G>T of TIM-3 gene polymorphisms might not play an important role as a genetic risk factor in the pathophysiology of ITP.
2. Mucin domain-containing molecule 3 (Tim-3) is preferentially expressed in lymphoma-derived endothelial cells.
3. Structural and biologic studies reveal that TIM-3 is a receptor for phosphatidylserine (PtdSer) and that TIM-3 polymorphic variants differ functionally in their recognition of PtdSer and clearance of apoptotic cells.
4. The TIM-3 pathway and the therapeutic potential of modulating the pathway in systemic lupus erythematosus, is reviewed.
5. haplotypes including TIM-3 genetic variants confer susceptibility to asthma in a Chinese population
6. Observational study of gene-disease association, gene-gene interaction, and gene-environment interaction. (HuGE Navigator)
7. Tim-3 expression correlates with a dysfunctional and senescent phenotype (CD127(low) CD57(high)), a central rather than effector memory profile (CD45RA(negative) CCR7(high)), and reduced Th1/Tc1 cytokine production.
8. These results suggest that TIM-3 is a negative regulator of human T cells and regulates Th1 and Th17 cytokine secretion.
9. Reduced TIM-3 expression in the lungs of patients may result in a defective T cell ability to control the Th1 immune response and could thus contribute to the pathogenesis of sarcoidosis.
10. The -1516G>T, -574T>G, and 4259G>T of TIM-3 gene polymorphisms might not play an important role as a genetic risk factor in the pathophysiology of ITP.
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Product Notes
The HAVCR2 havcr2 (Catalog #AAA606914) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3, TIM-3, TIMD3, FLJ14428, Hepatitis A Virus Cellular Receptor 2, HAVCR 2, HAVCR2, HAVCR-2, Kidney Injury Molecule 3, KIM-3) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tim3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated using whole blood monocytes. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of T5469-92F to up to 1x10e6 cells and incubate for 30 minutes at RT 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled rat IgG2A antibody. Researchers should empirically determine the suitability of the HAVCR2 havcr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tim3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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