Mouse anti-Human NFkB2 Monoclonal Antibody | anti-NFKB2 antibody

NFkB2 (Nuclear Factor kappa B)

Cat. Num. AAA605300

• Gene Names: NFKB2; p52; p105; H2TF1; LYT10; LYT-10; NF-kB2
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: NFkB2; Monoclonal Antibody; NFkB2 (Nuclear Factor kappa B); Anti -NFkB2 (Nuclear Factor kappa B); anti-NFKB2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7H260
• Specificity: Detects human NFkB2. Does not detect mouse NFkB2 in NIH 3T3 cells.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a powder lyophilized from a sterile-filtered solution in PBS containing 5% trehalose. Reconstitute to 0.5mg/ml with sterile PBS.

• Applicable Applications for anti-NFKB2 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ChIP, Immunocytochemistry and Western Blot.; Dilution: Chromatin Immunoprecipitation (ChIP): 5ug per 5x10e6 cells; Immunocytochemistry: 8-25ug/ml; Western Blot: 0.5ug/ml
• Immunogen: E. coli-derived NFkB2 (aa1-447)
• Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human Nuclear Factor kappa B2 (rhNF B2; aa 1-447: Accession # NP_002493).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NFKB2 antibody

Nuclear Factor kappa B2 (NF B2 or NF B p52) is a member of the NF B/Rel family of transcription factors. NF B2 dimerizes with other members of the NF B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Product Categories/Family for anti-NFKB2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 387157878
• NCBI GeneID: 4791
• NCBI Accession #: NP_001248332.1
• NCBI GenBank Nucleotide #: NM_001261403.1
• UniProt Accession #: Q00653; Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83; AAB23437
• Molecular Weight: 96,749 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform b
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• NCBI Official Symbol: NFKB2
• NCBI Official Synonym Symbols: p52; p105; H2TF1; LYT10; LYT-10; NF-kB2
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; DNA-binding factor KBF2; lymphocyte translocation chromosome 10 protein; nuclear factor of kappa light chain gene enhancer in B-cells 2
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: NFKB2
• UniProt Synonym Gene Names: LYT10; Lyt10
• UniProt Entry Name: NFKB2_HUMAN

NCBI Description

This gene encodes one of the subunits of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB transcription factor complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The NFkB complex can consist of different subunits that form both homo- or heterodimers which bind specific kappa-B elements in target genes. This gene encodes the p100 subunit that is processed into the active p52 subunit. This protein can function as both a transcriptional activator and repressor, depending on its dimer partner. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, May 2012]

Uniprot Description

NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protein type: Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; follicular dendritic cell differentiation; rhythmic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; positive regulation of interferon type I production; germinal center formation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway

Disease: Immunodeficiency, Common Variable, 10