Rabbit GRM8 Polyclonal Antibody | anti-Grm8 antibody

GRM8, CT (GRM8, GPRC1H, MGLUR8, Metabotropic glutamate receptor 8)

Cat. Num. AAA6000402

• Gene Names: Grm8; Gprc1h; mGluR8; A230002O04
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GRM8; Polyclonal Antibody; CT (GRM8; GPRC1H; MGLUR8; Metabotropic glutamate receptor 8); Anti -GRM8; anti-Grm8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GRM8/MGLUR8. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-Grm8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:10,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human GRM8/MGLUR8 at C-Terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(GRM8/MGLUR8 (10ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex neurons, followed by biotinylated goat, rabbit IgG, alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of extracts from mouse brain cells, using mGluR8. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-Grm8 antibody

Metabotropic Glutamate Receptor GRM8 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. Based on sequence homology, putative signal transduction mechanisms, and pharmacologic properties, GRM8 has been placed in Group III along with GRM4, GRM6, and GRM7. Group II and Group III receptors are linked to inhibition of the cyclic AMP signaling cascade, but differ in their agonist selectivities. Three isoforms of GRM8 are produced by alternative splicing.

Product Categories/Family for anti-Grm8 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 223459816
• NCBI GeneID: 14823
• UniProt Accession #: P47743; Q6B964
• Molecular Weight: 101,828 Da
• NCBI Official Full Name: Grm8 protein
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 8
• NCBI Official Symbol: Grm8
• NCBI Official Synonym Symbols: Gprc1h; mGluR8; A230002O04
• NCBI Protein Information: metabotropic glutamate receptor 8; G protein coupled receptor, family C, group 1, member H; G protein-coupled receptor, family C, group 1, member H
• UniProt Protein Name: Metabotropic glutamate receptor 8
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: Grm8
• UniProt Synonym Gene Names: Gprc1h; Mglur8; mGluR8
• UniProt Entry Name: GRM8_MOUSE

Uniprot Description

mGluR8: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Belongs to the G-protein coupled receptor 3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 3; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Cellular Component: presynaptic membrane; neuron projection; membrane; cell soma; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; signal transducer activity; group III metabotropic glutamate receptor activity; glutamate receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; detection of mechanical stimulus involved in sensory perception of pain; synaptic transmission; synaptic transmission, glutamatergic; negative regulation of cAMP biosynthetic process; regulation of synaptic transmission, glutamatergic; response to stimulus; sensory perception of smell; regulation of neurotransmitter secretion; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; signal transduction