Mouse anti-Bovine Transforming Growth Factor, beta Binding Protein 2, latent Monoclonal Antibody | anti-TGFB1I1 antibody

Transforming Growth Factor, beta Binding Protein 2, NT, latent (LTBP-2)

Cat. Num. AAA605150

• Gene Names: TGFB1I1; HIC5; ARA55; HIC-5; TSC-5
• Reactivity: Bovine
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Ascites; Ascites
• Synonyms: Transforming Growth Factor; beta Binding Protein 2; latent; Monoclonal Antibody; NT; latent (LTBP-2); Anti -Transforming Growth Factor; anti-TGFB1I1 antibody

• Host: Mouse
• Reactivity: Bovine
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G2
• Specificity: Recognizes bovine LTBP-2.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a lyophilized powder. Reconstitute with 200ul sterile ddH2O, 0.01% Thimerosal.

• Applicable Applications for anti-TGFB1I1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.; Dilution: ELISA: 1:1000 -1:2000; Western Blot: 1:200 -1:500; Immunoprecipitation: 1:100 -1:200; Immunohistochemistry: 1:50 -1:100; In many cases, antigenicity of elastic fiber components can be enhanced by treating with 6M guanidine HCl, 50mM dithiothreitol, 20mM Tris pH 8.0 for 15 minutes. After washing with 20mM Tris pH 8.0, the slides are treated with 100mM iodoacetamide in the dark for 15 minutes, washed and stained using normal protocols.
• Immunogen: Amino-terminal third of bovine LTBP-2.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.01% Thimerosal. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TGFB1I1 antibody

Elastic fibers play the principal roles in providing elasticity and integrity to various types of human organs, such as the arteries, lung, and skin. However, the molecular mechanism of elastic fiber assembly that leads to deposition and crosslinking of elastin along microfibrils remains largely unknown. It has been previously shown that developing arteries and neural crest EGF-like protein (DANCE) (also designated fibulin-5) is essential for elastogenesis by studying DANCE-deficient mice. It is reported that the identification of latent transforming growth factor--binding protein 2 (LTBP-2), an elastic fiber-associating protein whose function in elastogenesis is not clear, as a DANCE-binding protein. Elastogenesis assays using human skin fibroblasts reveal that fibrillar deposition of DANCE and elastin is largely dependent on fibrillin-1 microfibrils. However, downregulation of LTBP-2 induces fibrillin-1-independent fibrillar deposition of DANCE and elastin. Moreover, recombinant LTBP-2 promotes deposition of DANCE onto fibrillin-1 microfibrils. These results suggest a novel regulatory mechanism of elastic fiber assembly in which LTBP-2 regulates targeting of DANCE on suitable microfibrils to form elastic fibers.

Product Categories/Family for anti-TGFB1I1 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 150416155
• NCBI GeneID: 7041
• UniProt Accession #: O43294; Q9BPW3; Q9Y2V5; B2R8D5
• Molecular Weight: 49,814 Da
• NCBI Official Full Name: Transforming growth factor beta-1-induced transcript 1 protein
• NCBI Official Synonym Full Names: transforming growth factor beta 1 induced transcript 1
• NCBI Official Symbol: TGFB1I1
• NCBI Official Synonym Symbols: HIC5; ARA55; HIC-5; TSC-5
• NCBI Protein Information: transforming growth factor beta-1-induced transcript 1 protein; OTTHUMP00000163301; OTTHUMP00000197012; androgen receptor coactivator ARA55; hydrogen peroxide-inducible clone 5 protein; androgen receptor coactivator 55 kDa protein; androgen receptor-associated protein of 55 kDa
• UniProt Protein Name: Transforming growth factor beta-1-induced transcript 1 protein
• Protein Family: Transforming growth factor beta-1-induced transcript 1 protein
• UniProt Gene Name: TGFB1I1
• UniProt Synonym Gene Names: ARA55
• UniProt Entry Name: TGFI1_HUMAN

NCBI Description

This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Hic-5: Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA- binding activity. Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7. Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Up-regulated by TNF and hydrogen peroxide. Expressed in platelets, smooth muscle and prostate stromal cells. Belongs to the paxillin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular matrix; focal adhesion; nuclear matrix; cytoskeleton; cytoplasm; intracellular

Molecular Function: protein binding; Roundabout binding; androgen receptor binding; zinc ion binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; cell fate commitment; Wnt receptor signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of fat cell differentiation; ubiquitin-dependent SMAD protein catabolic process; negative regulation of cell proliferation; morphogenesis of embryonic epithelium; epithelial cell differentiation; response to heat; androgen receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; cell adhesion