Mouse anti-Human Dkk-3 Monoclonal Antibody | anti-DKK3 antibody

Dkk-3 (Dickkopf Homolog 3, REIC)

Cat. Num. AAA604539

• Gene Names: DKK3; RIG; REIC
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Dkk-3; Monoclonal Antibody; Dkk-3 (Dickkopf Homolog 3; REIC); Anti -Dkk-3 (Dickkopf Homolog 3; anti-DKK3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 7H46
• Specificity: Recognizes human Dkk-3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.

• Applicable Applications for anti-DKK3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1-2ug/ml (non-reducing conditions)
• Immunogen: NS0-derived recombinant human Dickkopf homolog 3
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DKK3 antibody

Dkk-3, also known as REIC (Reduced Expansion in Immortalized Cells), is a secreted protein of the Dickkopf family. It is reported to have antiproliferative activity and is down-regulated in some tumors.

Product Categories/Family for anti-DKK3 antibody

Antibodies; Abs to Dickkopf Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 66346689
• NCBI GeneID: 27122
• NCBI Accession #: NP_001018067.1
• NCBI GenBank Nucleotide #: NM_001018057.1
• UniProt Accession #: Q6PQ81; Q9UBP4
• Molecular Weight: 38,340 Da
• NCBI Official Full Name: dickkopf-related protein 3
• NCBI Official Synonym Full Names: dickkopf homolog 3 (Xenopus laevis)
• NCBI Official Symbol: DKK3
• NCBI Official Synonym Symbols: RIG; REIC
• NCBI Protein Information: dickkopf-related protein 3; dkk-3; hDkk-3; dickkopf 3; dickkopf-3; RIG-like 5-6; RIG-like 7-1; OTTHUMP00000230783; OTTHUMP00000230784; OTTHUMP00000230785; OTTHUMP00000230786; regulated in glioma
• UniProt Protein Name: Putative tumor suppressor Dkk-3/REIC
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK3
• UniProt Entry Name: Q6PQ81_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Uniprot Description

DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular space

Biological Process: anatomical structure morphogenesis; Wnt receptor signaling pathway; adrenal gland development; negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent