Rat anti-Mouse Meteorin Monoclonal Antibody | anti-METRN antibody

Meteorin

Cat. Num. AAA603999

• Gene Names: METRN; MGC2601; C16orf23; c380A1.2
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Meteorin; Monoclonal Antibody; Anti -Meteorin; anti-METRN antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 7H112
• Specificity: Recognizes mouse Meteorin.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, dH2O PBS.

• Applicable Applications for anti-METRN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and Direct ELISA.; Dilution: Western Blot: 1-2ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.; Direct ELISA: 0.5-1ug/ml. The detection limit is ~50 ng/well.; Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: Recombinant mouse Meteorin.
• Hybridoma: Mouse myeloma cells were fused with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Meteorin.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-METRN antibody

Meteorin is a novel 31kD, secreted protein that is expressed by undifferentiated neural and glial progenitors, and astrocytes. Its name derives from it ability to generate glial cells with long meteor-like tails. Mouse meteorin is synthesized as a 291 amino acid (aa) precursor that contains a 270 aa mature region. It contains eight cysteines with no identifiable domains or N-linked glycosylation sites. There is one alternate splice form where the first 164 aa of the precursor are deleted and aa # 165 is changed to a Met, possibly to serve in translation initiation. Mature mouse meteorin is 82% and 77% aa identical to human and bovine meteorin, respectively.

Product Categories/Family for anti-METRN antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13129000
• NCBI GeneID: 79006
• NCBI Accession #: NP_076947.1
• NCBI GenBank Nucleotide #: NM_024042.2
• UniProt Accession #: Q9UJH8; Q9UJH9
• Molecular Weight: 31,207 Da
• NCBI Official Full Name: meteorin
• NCBI Official Synonym Full Names: meteorin, glial cell differentiation regulator
• NCBI Official Symbol: METRN
• NCBI Official Synonym Symbols: MGC2601; C16orf23; c380A1.2
• NCBI Protein Information: meteorin; OTTHUMP00000045518
• UniProt Protein Name: Meteorin
• Protein Family: Meteorin
• UniProt Gene Name: METRN
• UniProt Synonym Gene Names: C16orf23
• UniProt Entry Name: METRN_HUMAN

NCBI Description

Meteorin regulates glial cell differentiation and promotes the formation of axonal networks during neurogenesis (Nishino et al., 2004 [PubMed 15085178]).[supplied by OMIM]

Uniprot Description

METRN: Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia. Belongs to the meteorin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular space

Biological Process: glial cell differentiation; positive regulation of axonogenesis