Mouse p120 ctn Monoclonal Antibody | anti-NOP2 antibody

p120 ctn, NT

Cat. Num. AAA603684

• Gene Names: NOP2; NOL1; p120; NSUN1; NOP120
• Reactivity: Canine, Chicken, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Serum; Neat serum
• Synonyms: p120 ctn; Monoclonal Antibody; NT; Anti -p120 ctn; anti-NOP2 antibody

• Host: Mouse
• Reactivity: Canine, Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G229
• Specificity: Specific for isoforms 1 and 2 of p120ctn. Crossreactivity with p120ctn isoforms 3 and 4 has not been observed. Reactivity of this antibody has been confirmed by Western blotting, immunoprecipitation and immunofluorescence.(17). Species crossreactivity: human, mouse, rat and canine and chicken p120ctn. Positive control lysates include MDCK (canine) cells, NIH 3T3 (mouse) cells, Hela (human) cells, Va-2 fibroblasts (human), rat-2 fibroblasts and chicken embryo fibroblasts.
• Purity/Purification: Serum; Neat serum
• Form/Format: Supplied as a liquid of neat (undiluted) mouse ascites fluid, 0.05% sodium azide as a preservative.

• Applicable Applications for anti-NOP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in mmunofluorescence, ELISA, Western Blotting, Immunoprecipitation.; Dilution: Immunofluorescence: 1:250-1:500; ELISA: 1:1000-1:10,000; Western Blotting: 1:1000; Immunoprecipitation: 5-10 ml/IP reaction
• Immunogen: Recombinant fusion protein derived from the N-terminus of murine p120ctn.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NOP2 antibody

Originally characterized as a prominent substrate for Src(14) and receptor protein tyrosine kinases (eg, EGF, PDGF, CSF- 1)(6), p120ctn is a novel catenin (10, 13) which binds directly to the juxtamembrane region of classical cadherins (eg, Ecadherin).(3, 11, 13, 18) Emerging evidence suggests a prominent role in the regulation of cadherin mediated adhesion.(1, 4, 12) Most cells express multiple isoforms of p120 that arise by alternative splicing(8, 9, 11), some of which can be distinguished using monoclonal antibodies.(17) Fibroblasts express mostly type 1 isoforms containing an N-terminal extension of 101 amino acids. Type I isoforms may be important for cell motility and are selectively recognized by Mab 6H11. Epithelial cells preferentially express shorter isoforms lacking this extension. Mab 15D2 reacts with a C-terminal epitope found in all known p120 isoforms. Heterogeneous or negative p120 staining has been reported in breast(5) and colon carcinomas(7, 16), and decreased p120 expression in bladder tumors correlated with a poor prognosis.(15) Loss of p120 or E-cadherin never occurs in epithelial tissues under normal circumstances, and its expression is probably mandatory for normal tissue architecture. Interestingly, p120 was recently reported to bind a nuclear POZ/ZF transcription factor called Kaiso.(2) Thus, like its cousin b-catenin, p120 may have dual roles in both cell-cell adhesion and in nuclear signaling.

Product Categories/Family for anti-NOP2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 385198063
• NCBI GeneID: 4839
• NCBI Accession #: NP_001245239.1
• NCBI GenBank Nucleotide #: NM_001258310.1
• UniProt Accession #: P46087; Q05BA7; Q0P5S5; Q3KQS4; Q58F30; A1A4Z3; B3KPD6
• Molecular Weight: 89,302 Da
• NCBI Official Full Name: putative ribosomal RNA methyltransferase NOP2 isoform 4
• NCBI Official Synonym Full Names: NOP2 nucleolar protein
• NCBI Official Symbol: NOP2
• NCBI Official Synonym Symbols: NOL1; p120; NSUN1; NOP120
• NCBI Protein Information: putative ribosomal RNA methyltransferase NOP2; nucleolar protein 1, 120kDa; nucleolar protein 2 homolog; NOP2 nucleolar protein homolog; NOL1/NOP2/Sun domain family, member 1; proliferating-cell nucleolar antigen p120; proliferation-associated nucleolar protein p120
• UniProt Protein Name: Putative ribosomal RNA methyltransferase NOP2
• Protein Family: Probable 28S rRNA
• UniProt Gene Name: NOP2
• UniProt Synonym Gene Names: NOL1
• UniProt Entry Name: NOP2_HUMAN

Uniprot Description

NOP2: May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation. May act as ribosomal RNA methyltransferase. Belongs to the methyltransferase superfamily. RsmB/NOP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; EC 2.1.1.-; Cell cycle regulation; Nucleolus; Methyltransferase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nucleolus

Molecular Function: protein binding; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: methylation; positive regulation of cell proliferation; rRNA processing