Mouse anti-Human, Mouse MAP Kinase p38, beta Monoclonal Antibody | anti-Mapk14 antibody

MAP Kinase p38, beta (Mitogen-activated Protein Kinase p38 beta, MAP Kinase p38 beta, p38beta, P38beta2, p38b, Mitogen-activated Protein Kinase 11, MAP Kinase 11, MAPK 11, MAPK11, PRKM11, p38-2, Stress-activated Protein Kinase 2b, SAPK2b, SAPK2)

Cat. Num. AAA603100

• Gene Names: Mapk14; RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Purified; Purified from mouse ascites.
• Synonyms: MAP Kinase p38; beta; Monoclonal Antibody; beta (Mitogen-activated Protein Kinase p38 beta; MAP Kinase p38 beta; p38beta; P38beta2; p38b; Mitogen-activated Protein Kinase 11; MAP Kinase 11; MAPK 11; MAPK11; PRKM11; p38-2; Stress-activated Protein Kinase 2b; SAPK2b; SAPK2); Anti -MAP Kinase p38; anti-Mapk14 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G181
• Specificity: This monoclonal antibody is specific for the beta-isoform of p38. Cross-reactivity with other p38 isoforms (including p38-a,-d and-g) has not been observed.
• Purity/Purification: Purified; Purified from mouse ascites.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-Mapk14 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blotting, Immunoprecipitation.; Dilution: ELISA: 0.1 - 1.0ug/ml; Western Blotting: 1-3ug/ml; Immunoprecipitation: 5-10ug/IP reaction
• Immunogen: Recombinant, full-length human p38 2; the product of the p38-b gene
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Mapk14 antibody

p38 (CSBP, RK, or Mpk2) is among the newest MAPK family members to be isolated. This protein was originally identified as a kinase which was phosphorylated on tyrosine in response to treatment of cells with LPS. Subsequently, p38 (CSBP1/CSBP2) was identified as the target of pyridinyl-imidazole compounds (eg: SB 203580) which inhibit the production of interleukin-1 and tumor necrosis factor from stimulated human monocytes. CSBP1 and CSBP2 are splice variants of p38 differing only in an internal 25 amino acid sequence. p38 and JNKs appear to be activated by many of the same stimuli; nevertheless, differences in the extent and timing of activation of p38 and JNK have been observed. Further, p38 and JNKS are activated by distinct MAPK kinases. For example, MKK4 activates both JNK and p38, whereas MKK3 and MKK6 activate p38 specifically. Studies have implicated p38 in the phosphorylation of the small heat shock proteins Hsp27, MAPKAP kinase-2 and-3, and the transcription factors ATF2, Elk-1 and CHOP. In addition, p38 likely mediates increased cytokine expression and some aspects of programmed cell death.

Product Categories/Family for anti-Mapk14 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 62461582
• NCBI GeneID: 81649
• NCBI Accession #: NP_112282.2
• NCBI GenBank Nucleotide #: NM_031020.2
• UniProt Accession #: P70618; O08594; Q99MG4
• Molecular Weight: 41,321 Da
• NCBI Official Full Name: mitogen-activated protein kinase 14
• NCBI Official Synonym Full Names: mitogen activated protein kinase 14
• NCBI Official Symbol: Mapk14
• NCBI Official Synonym Symbols: RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• NCBI Protein Information: mitogen-activated protein kinase 14; MAPK 14; MAP kinase 2; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; reactive kinase; MAP kinase p38 alpha; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; mitogen-activated protein kinase 14A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine-supressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: Mapk14
• UniProt Synonym Gene Names: Csbp1; Csbp2; MAP kinase 14; MAPK 14
• UniProt Entry Name: MK14_RAT

NCBI Description

mitogen-activated protein kinase; involved in intracellular signalling, inhibition of apoptosis and gene activation [RGD, Feb 2006]

Uniprot Description

P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily

Cellular Component: nucleoplasm; spindle pole; mitochondrion; cell; cytoplasm; cytosol; nucleus

Molecular Function: protein C-terminus binding; MAP kinase activity; protein serine/threonine kinase activity; protein binding; NFAT protein binding; kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; protein amino acid autophosphorylation; cell morphogenesis; stress-activated MAPK cascade; response to lipopolysaccharide; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of glucose import; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; response to glucose stimulus; chondrocyte differentiation; angiogenesis; placenta development; positive regulation of cardiac muscle cell proliferation; cartilage condensation; skeletal muscle development; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; positive regulation of blood vessel endothelial cell migration; MAPKKK cascade; DNA damage response, signal transduction; glucose metabolic process; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; response to muramyl dipeptide; DNA damage checkpoint; striated muscle cell differentiation; fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus; myoblast cell differentiation involved in skeletal muscle regeneration