Rabbit anti-Human MAP Kinase p38, beta Polyclonal Antibody | anti-Mapk14 antibody

MAP Kinase p38, beta (Mitogen-activated Protein Kinase p38 beta, MAP Kinase p38 beta, p38beta, P38beta2, p38b, Mitogen-activated Protein Kinase 11, MAP Kinase 11, MAPK 11, MAPK11, PRKM11, p38-2, Stress-activated Protein Kinase 2b, SAPK2b, SAPK2)

Cat. Num. AAA628289

• Gene Names: Mapk14; RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: MAP Kinase p38; beta; Polyclonal Antibody; beta (Mitogen-activated Protein Kinase p38 beta; MAP Kinase p38 beta; p38beta; P38beta2; p38b; Mitogen-activated Protein Kinase 11; MAP Kinase 11; MAPK 11; MAPK11; PRKM11; p38-2; Stress-activated Protein Kinase 2b; SAPK2b; SAPK2); Anti -MAP Kinase p38; anti-Mapk14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human p38 beta.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Mapk14 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the center region of human P38beta (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CTDSP1 (arrow) using rabbit polyclonal p38 beta Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTDSP1 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(P38beta Antibody (Ctr) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the P38beta antibody detected the P38beta protein (arrow).)

Related Product Information for anti-Mapk14 antibody

P38 beta is a member of the MAP kinase subfamily of Ser/Thr protein kinases. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is closely related to p38 MAP kinase, both of which can be activated by changes in osmolarity of the extracellular environment, proinflammatory cytokines and cellular stress. This protein preferentially phosphorylates transcription factor ATF2. It is activated by phosphorylation on threonine and tyrosine by MKK6, and inhibited by pyridinyl-imidazole related compounds. Highest levels of expression are found in the brain and heart, with additional expression in the placenta, lung, liver, skeletal muscle, kidney and pancreas.

Product Categories/Family for anti-Mapk14 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 62461582
• NCBI GeneID: 81649
• NCBI Accession #: NP_112282.2
• NCBI GenBank Nucleotide #: NM_031020.2
• UniProt Accession #: P70618; O08594; Q99MG4
• Molecular Weight: 41,321 Da
• NCBI Official Full Name: mitogen-activated protein kinase 14
• NCBI Official Synonym Full Names: mitogen activated protein kinase 14
• NCBI Official Symbol: Mapk14
• NCBI Official Synonym Symbols: RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• NCBI Protein Information: mitogen-activated protein kinase 14; MAPK 14; MAP kinase 2; MAP kinase 14; p38alpha Exip; p38 MAP kinase; MAP kinase Mxi2; reactive kinase; MAP kinase p38 alpha; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; mitogen-activated protein kinase 14A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine-supressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: Mapk14
• UniProt Synonym Gene Names: Csbp1; Csbp2; MAP kinase 14; MAPK 14
• UniProt Entry Name: MK14_RAT

NCBI Description

mitogen-activated protein kinase; involved in intracellular signalling, inhibition of apoptosis and gene activation [RGD, Feb 2006]

Uniprot Description

P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis

Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily

Cellular Component: nucleoplasm; spindle pole; mitochondrion; cell; cytoplasm; cytosol; nucleus

Molecular Function: protein C-terminus binding; MAP kinase activity; protein serine/threonine kinase activity; protein binding; NFAT protein binding; kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; protein amino acid autophosphorylation; stress-activated MAPK cascade; cell morphogenesis; response to lipopolysaccharide; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of glucose import; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; response to glucose stimulus; lipopolysaccharide-mediated signaling pathway; chondrocyte differentiation; angiogenesis; placenta development; positive regulation of cardiac muscle cell proliferation; cartilage condensation; skeletal muscle development; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; positive regulation of blood vessel endothelial cell migration; MAPKKK cascade; DNA damage response, signal transduction; glucose metabolic process; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; response to muramyl dipeptide; DNA damage checkpoint; striated muscle cell differentiation; fatty acid oxidation; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus; myoblast cell differentiation involved in skeletal muscle regeneration