Human Mitogen-activated protein kinase 14 ELISA Kit | MAPK14 elisa kit

Human Mitogen-activated protein kinase 14 ELISA Kit

Cat. Num. AAA765615

• Gene Names: Mapk14; RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• Reactivity: Human
• Synonyms: Mitogen-activated protein kinase 14; Human Mitogen-activated protein kinase 14 ELISA Kit; MAPK14/SAPK2A/SAPK2a/Mitogen-activated protein kinase p38 alpha/MAP kinase p38 alpha/MAX-interacting protein 2/Cytokine suppressive anti-inflammatory drug-binding protein/CSAID-binding protein/CSBP/MAP kinase MXI2; MAPK14 elisa kit

• Reactivity: Human
• Sequence Length: 360

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MAPK14 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MAPK14 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MAPK14 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MAPK14 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MAPK14 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 62461582
• NCBI GeneID: 81649
• NCBI Accession #: NP_112282.2
• NCBI GenBank Nucleotide #: NM_031020.2
• Molecular Weight: 41,521 Da
• NCBI Official Full Name: mitogen-activated protein kinase 14
• NCBI Official Synonym Full Names: mitogen activated protein kinase 14
• NCBI Official Symbol: Mapk14
• NCBI Official Synonym Symbols: RK; Hog; p38; CRK1; CSBP; Exip; Mxi2; CSPB1; Csbp1; Csbp2; Prkm14; Prkm15; Sapk2A; p38Hog; p38alpha
• NCBI Protein Information: mitogen-activated protein kinase 14
• UniProt Protein Name: Mitogen-activated protein kinase 14
• Protein Family: Mitogen-activated protein kinase
• UniProt Gene Name: Mapk14
• UniProt Synonym Gene Names: Csbp1; Csbp2; MAP kinase 14; MAPK 14; MAP kinase p38 alpha
• UniProt Entry Name: MK14_RAT

NCBI Description

mitogen-activated protein kinase; involved in intracellular signalling, inhibition of apoptosis and gene activation [RGD, Feb 2006]

Uniprot Description

P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; p38 subfamily; MAPK/p38 subfamily

Cellular Component: cell; cytoplasm; cytosol; intracellular; mitochondrion; nucleoplasm; nucleus; spindle pole

Molecular Function: ATP binding; enzyme binding; kinase activity; MAP kinase activity; NFAT protein binding; protein binding; protein C-terminus binding; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity

Biological Process: angiogenesis; apoptosis; cartilage condensation; cell morphogenesis; chondrocyte differentiation; DNA damage checkpoint; DNA damage response, signal transduction; fatty acid oxidation; glucose metabolic process; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; myoblast cell differentiation involved in skeletal muscle regeneration; osteoclast differentiation; peptidyl-serine phosphorylation; placenta development; positive regulation of blood vessel endothelial cell migration; positive regulation of cardiac muscle cell proliferation; positive regulation of cyclase activity; positive regulation of erythrocyte differentiation; positive regulation of glucose import; positive regulation of myoblast differentiation; positive regulation of protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of ossification; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to glucose stimulus; response to lipopolysaccharide; response to muramyl dipeptide; skeletal muscle development; stress-activated MAPK cascade; striated muscle cell differentiation; transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; vascular endothelial growth factor receptor signaling pathway