Mouse anti-Human Desmocollin-2,3 Monoclonal Antibody | anti-DSC2 antibody

Desmocollin-2,3

Cat. Num. AAA602772

• Gene Names: DSC2; DG2; DSC3; CDHF2; ARVD11; DGII/III; DKFZp686I11137
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Desmocollin-2; 3; Monoclonal Antibody; Anti -Desmocollin-2; anti-DSC2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G130
• Specificity: Recognizes the ~120kD desmocollin-2 and ~105kD desmocollin-3 human protein, with particularly strong reaction to desmocollin-2. Crossreactivity with other desmocollin family members has not been observed.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-DSC2 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence., Recommended Dilutions:, Immunoprecipitation (Native): 5-10ug, Western Blot (ECL): 1-3ug/ml, Immuno uorescence: 20-50ug/ml, Optimal dilutions to be determined by the researcher., Recommended Positive Control:, A0001-50: A431 Cells, Non-Stimulated
• Immunogen: Human desmocollin-2 extracellular domain
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-DSC2 antibody

Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein1-3 and desmocollin1-3. Desmosomal cadherins showed differentiation- specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers. Domain specific desmosomal and adherens junction-associated antibodies have been widely used in recent studies. In one study, domain specific antibodies were used to determine the cellular localization of the skin of patients with Hailey-Hailey disease and Darier's disease. Desmosomal cadherin antibodies are also useful for targeting specific disease and in the study of interaction between cell adhesion proteins.

Product Categories/Family for anti-DSC2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13435366
• NCBI GeneID: 1824
• NCBI Accession #: NP_004940.1
• NCBI GenBank Nucleotide #: NM_004949.3
• UniProt Accession #: Q02487; Q63HM4; Q68DY8
• Molecular Weight: 99,962 Da
• NCBI Official Full Name: desmocollin-2 isoform Dsc2b preproprotein
• NCBI Official Synonym Full Names: desmocollin 2
• NCBI Official Symbol: DSC2
• NCBI Official Synonym Symbols: DG2; DSC3; CDHF2; ARVD11; DGII/III; DKFZp686I11137
• NCBI Protein Information: desmocollin-2; OTTHUMP00000162822; OTTHUMP00000162823; cadherin family member 2; desmosomal glycoprotein II; desmosomal glycoprotein III; desmosomal glycoprotein II/III
• UniProt Protein Name: Desmocollin-2
• Protein Family: Desmocollin
• UniProt Gene Name: DSC2
• UniProt Synonym Gene Names: CDHF2; DSC3
• UniProt Entry Name: DSC2_HUMAN

NCBI Description

The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11. Alternative splicing results in two transcript variants encoding distinct isoforms. [provided by RefSeq]

Uniprot Description

DSC2: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Defects in DSC2 are the cause of familial arrhythmogenic right ventricular dysplasia type 11 (ARVD11); also known as arrhythmogenic right ventricular cardiomyopathy 11 (ARVC11). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; cell-cell adherens junction; plasma membrane; integral to membrane; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding

Biological Process: homophilic cell adhesion; cell adhesion; cellular response to starvation

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 11